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A kind of growth-promoting cultivation method of Hubei Fritillaria

A cultivation method and technology of Fritillaria, which is applied in the field of rapid propagation of Fritillaria chinensis, can solve the problems of less research on Fritillaria chinensis and no breeding system, and achieve the effects of improving survival rate, utilization rate and soil fertility

Inactive Publication Date: 2016-04-27
SUZHOU RENCHENG BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been a lot of researches on Fritillaria tissue culture and rapid propagation technology, but there are relatively few studies on Hubei Fritillaria, and a complete breeding system has not been formed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A growth-promoting cultivation method of Hubei Fritillaria, comprising the following steps:

[0026] (1) Selection and disinfection of explants: firstly select vigorous and mature fresh Hubei Fritillaria bulbs, peel off the outermost dry and wrinkled scales of the bulbs and the residual old roots at the base, and rinse them twice with running water , then soaked in 50% rifampicin 300 times solution for 20min, blotted dry with filter paper, then peeled off the bulbs layer by layer, and then transferred the peeled scales into a high-pressure sterilized beaker, placed in an ultra-clean Soak and sterilize the workbench with 75% alcohol for 30 seconds, remove the alcohol, rinse with sterile water for 3 times, then disinfect with 2% sodium hypochlorite solution for 10 minutes, and rinse with sterile water twice. Quickly add 3% hydrogen peroxide to soak and sterilize for 8 minutes, shake the beaker frequently during the soaking process, then pour out 1 liter of hydrogen peroxi...

Embodiment 2

[0035] A growth-promoting cultivation method of Hubei Fritillaria, comprising the following steps:

[0036] (1) Selection and disinfection of explants: firstly select vigorous and mature fresh Hubei Fritillaria bulbs, peel off the outermost dry and wrinkled scales of the bulbs and the residual old roots at the base, and rinse them with running water for 3 times , then soaked in 50% rifampicin 300 times solution for 30min, blotted dry with filter paper, then peeled off the bulbs layer by layer, and then transferred the peeled scales into a high-pressure sterilized beaker, placed in an ultra-clean Soak and sterilize the workbench with 75% alcohol for 30 seconds, remove the alcohol, rinse with sterile water for 4 times, then disinfect with 2% sodium hypochlorite solution for 10 minutes, and rinse with sterile water for 3 times. Quickly add 3% hydrogen peroxide to soak and sterilize for 10 minutes, shake the beaker frequently during the soaking process, then pour out 1 liter of hy...

Embodiment 3

[0044] A growth-promoting cultivation method of Hubei Fritillaria, characterized in that it comprises the following steps:

[0045] (1) Selection and disinfection of explants: firstly, select fresh, robust and mature Hubei fritillary bulbs, peel off the dry and wrinkled scales on the outermost layer of the bulbs and the residual old roots at the base, and rinse them with running water for 2- 3 times, then soak in 50% rifampicin 300 times solution for 20-30min, then dry the water with filter paper, then peel off the bulbs layer by layer, and then transfer the peeled scales into a high-pressure sterilized beaker , soak and sterilize with 75% alcohol for 30s on the ultra-clean workbench, remove the alcohol, rinse with sterile water for 3-4 times, then disinfect with 2% sodium hypochlorite solution for 10 minutes, and rinse with sterile water for 2-3 times. Quickly add 3% hydrogen peroxide to soak and sterilize for 8-10 minutes, shake the beaker frequently during the soaking proce...

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PUM

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Abstract

The invention discloses a growth-promoting cultivation method of Hubei Fritillaria, comprising selection and disinfection treatment of explants, material treatment, callus induction and bud differentiation, bud proliferation, subculture of callus, rooting Cultivation, greenhouse seedling hardening, soil management, transplanting and other steps, after the above treatments, the callus formation rate reached more than 96%, the multiplication multiple exceeded 7.3 times, the rooting rate reached more than 98%, and the final transplanting survival rate It has reached more than 98%, and the plants grow vigorously, which improves the soil fertility and disease control rate. And in the cultivation process, the proliferation of buds and the proliferation of callus are treated separately, which improves the utilization rate of materials and the survival rate of both.

Description

technical field [0001] The invention relates to a tissue culture method of Fritillaria, in particular to a rapid propagation method of Hubei Fritillaria. Background technique [0002] Fritillaria hupehensis Hsiaoet K.C.Hsia, also known as Ebei, Banbei, Kiln Shell, Fengbei, is a perennial herb of the genus Fritillaria L. in the family Liliaceae. Hubei Fritillaria was established in Hubei, Xuan It is cultivated in the area of ​​Enshi, and it is now the variety of Enshi GAP base. It is one of the medicinal materials vigorously developed in Enshi Prefecture. At present, there are widespread problems of environmental deterioration and lack of soil nutrients. The reproduction of Hubei Fritillaria is mainly based on bulbs, and scales can also be used. And seed reproduction, after several generations of asexual reproduction, sexual reproduction should be carried out once. However, because Hubei Fritillaria blooms a lot, the results are very few, and it is difficult to receive more ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 吴志刚
Owner SUZHOU RENCHENG BIO SCI & TECH
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