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Method for improving agar content of gracilaria lemaneiformis

A technology of asparagus and content, which is applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problems of low asparagus, short cultivation time, poor high temperature and low temperature resistance, and the like, and achieves growth. The effect of increasing the speed and increasing the content of agar

Active Publication Date: 2013-12-25
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the agar content of asparagus is still low, and the agar content is generally about 18% (dry weight). At the same time, asparagus has poor resistance to high and low temperatures, so it can only spend summer in the north and winter in the south. shorter time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] In the sea area where the maximum temperature is 30-35°C during the day, asparagus that is damaged by high temperature, put the asparagus cultivated in the sea area into the phytohormone culture solution for the first culture for 8-12 hours at night, and then the asparagus The vegetables are temporarily raised in the sea area, and then put the asparagus in the phytohormone culture solution for secondary cultivation at night for 8 to 12 hours, and then put it back to the sea area for cultivation, and then put the asparagus in the plant at night at an interval of 2 days. Carry out three times of cultivation in the hormone culture solution for 8-12 hours, put it back into the sea area for cultivation, and at an interval of 2 days, put the asparagus in the plant hormone culture solution for four times of cultivation for 8-12 hours at night, and put it back into the sea area for cultivation. The concentration of 2,4-epibrassinolide in the plant hormone culture solution is 0.1...

Embodiment 2

[0014] It is basically the same as the embodiment, except that the minimum daytime temperature is 15° C. in the sea area, and the asparagus damaged by low temperature is treated with phytohormone culture solution for four times and cultivated on the 9th day, and the results shown in Table 2 are obtained;

[0015] Table 2: Effects on Asparagus agar content and growth rate of low temperature stress in Example 2

[0016] control group 0.1 mg / L treatment 0.5 mg / L treatment 1.0 mg / L treatment relative growth rate 3.12±0.24 3.78±0.40 4.36±0.20 3.61±0.12 Agar content (%) 18.07±0.21 20.87±0.50 21.65±0.69 19.47±0.53

[0017] It can be seen from Table 2 that after four treatments of phytohormone culture solution, the highest content of asparagus in the treatment group can reach 21.65% of the dry weight of asparagus (2,4-epibrassinolide concentration 0.5 mg / L), The control group was 18.07%. In addition, the low temperature resistance of the aspar...

Embodiment 3

[0019] It is basically the same as the embodiment, except that the maximum daytime temperature is 25°C sea area, and the uninjured asparagus is cultivated on the 9th day after being treated with phytohormone culture solution for four times, and the results shown in Table 3 are obtained;

[0020] Table 3: Effects on Asparagus agar content and growth rate at normal temperature in Example 3

[0021] control group 0.1 mg / L treatment 0.5 mg / L treatment 1.0 mg / L treatment relative growth rate 5.78±0.20 6.16±0.27 6.48±0.43 5.95±0.38 Agar content (%) 18.27±0.12 20.6±0.53 20.93±0.42 19.67±0.32

[0022] As can be seen from Table 3, after four treatments of phytohormone culture solution, the highest content of asparagus agar in the treatment group can reach 20.93% of the dry weight of asparagus (2,4-epibrassinolide concentration 0.5 mg / L ), the control group was 18.27%. Its relative growth rate was 6.48, while that of the control group was only 5...

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Abstract

The invention provides a method for improving agar content of gracilaria lemaneiformis. The method includes: placing the gracilaria lemaneiformis cultivated in a sea area into plant hormone culture solution at night for first cultivation prior to temporary cultivation in the sea area, placing the gracilaria lemaneiformis into the plant hormone culture solution at the same night for second cultivation prior to placing back in the sea area for temporary cultivation, and then at an interval of two days, placing the gracilaria lemaneiformis into the plant hormone culture solution for third cultivation and the fourth cultivation prior to placing back to the sea area for cultivation. The plant hormone culture solution is 2,4-epibrassinolide solution 0.1-1.0mg / L in concentration, and the 2,4-epibrassinolide solution is prepared by firstly dissolving 2,4-epibrassinolide into ethyl alcohol 70-80% in volume concentration to prepare mother solution 1000mg / L in 2,4-epibrassinolide concentration and finally adding clean sea water. After nine days of cultivation of the plant hormone culture solution for four times, the agar content of the gracilaria lemaneiformis can reach a maximum of 21.65%.

Description

technical field [0001] The invention relates to a cultivation method of asparagus, in particular to a method for increasing the agar content of asparagus. Background technique [0002] Gracilaria( Gracilaria lemaneiformis ) is an important large economical seaweed, mainly used for extracting agar and as bait for rare seafood such as abalone. Asparagus is native to the Shandong Peninsula. The most suitable water temperature for growth of asparagus is 18-26°C. The growth of asparagus is restricted under the condition of too high or too low temperature, which affects the large-scale cultivation of asparagus. The successful selection and breeding of high-temperature-resistant asparagus, such as the invention patent application with the publication number CN101564004, discloses that asparagus is mutated by MNNG, and then cultivated at high temperature in HYP to obtain high-temperature-resistant asparagus. Large-scale cultivation of asparagus is also carried out in the sea areas...

Claims

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Application Information

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IPC IPC(8): A01G33/00
Inventor 孙雪李静徐年军王俏俏
Owner NINGBO UNIV