Use of tabancus yao macquart antithrombotic polypeptide vasotab TY
An anti-thrombosis and platelet aggregation technology, applied in the field of biomedicine, can solve problems such as no public reports, and achieve the effect of no bleeding side effects
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Embodiment 1
[0023] Example 1: The vasodilatory effect of the antithrombotic peptide vasotab TY
[0024] Male Wistar rats (180-200g) were sacrificed by force, and the femoral artery was stripped off, and a 10mm-long femoral artery was cut off and placed in K-Linger's bicarbonate solution (118mM NaCl, 5mM KCl, 2.5mM CaCl 2 ,1.2mM MgSO 4 .7H 2 O, 1.2mM KH 2 PO 4 , 1mM EDTA, 1.1mM ascorbic acid, 11mM glucose), remove the connective tissue and fat, remove the vascular endothelium with a cotton swab, make a 2-3mm long arterial ring, hang it in the vascular groove of the HV-4 vascular ring perfusion system, and injected into the small bath with 37°C Kling’s buffer, and filled with 95% O 2 +5%CO 2 The mixed gas is saturated with oxygen. It is connected to the BL-420E biological function experiment system through the data line, and the blood vessel tension value is converted into a data signal. The vascular ring was repeatedly washed with normal K-Linds buffer until the tension was 2.0 g, t...
Embodiment 2
[0026] Example 2: Inhibitory effect of Yaofly antithrombotic peptide vasotab TY on platelet aggregation
[0027] Platelets from healthy people were diluted with plasma to 2.5×10 8 pieces / ml. Take 300 μl of plasma-rich platelets, add an appropriate amount of samples and incubate for 5 minutes, add 2 mmol / L ADP to induce aggregation, and draw the aggregation curve within 5 minutes on a platelet aggregometer. Platelet aggregation without sample treatment was used as a control.
[0028] Calculation of aggregation inhibition rate (I):
[0029] I=B / A×100%
[0030] A = the maximum aggregation that can be achieved by the agonist (expressed in light transmittance);
[0031] B = maximum aggregation (expressed in light transmittance) that can be achieved by adding agonist after sample exposure.
[0032] like figure 2 As shown, the antithrombotic polypeptide vasotab TY inhibited platelet aggregation induced by ADP in a gradient-dependent manner, 4.8 μg / ml antithrombotic polypeptide...
Embodiment 3
[0033] Example 3: Antithrombotic effect of Yaofly antithrombotic polypeptide vasotab TY in rat arteriovenous bypass model experiment
[0034] Male Wistar rats (180-200g, 9 in each group) were anesthetized by intraperitoneal injection of ketamine (50mg / kg) and xylazine (15mg / kg), and the skin and muscles of the neck were peeled off, and a trifurcated shunt catheter (two of them) was inserted. A polyethylene tube (PE-60) with a length of 10 cm is connected to the arteries and veins respectively, and a polyethylene tube with a length of 3 cm (PE-160)) in the middle, and a rough nylon filament folded into two sections (6 cm long and 0.26 mm in diameter) was placed in the middle polyethylene tube. The shunt was opened 15 minutes after the femoral vein was stripped and the shunt was closed after 5 minutes, and the nylon wire was removed and weighed.
[0035] The result is as image 3 As shown in A, the weight of the thrombus in the negative control saline group was 7±0.6 mg. After...
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