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Application of yeast-origin glucan on preparation of drugs for curing hepatitis B virus infection

A technology for hepatitis B virus and hepatitis B virus, which can be used in antiviral agents, drug combinations, pharmaceutical formulations, etc., to solve problems such as heavy economic burden and strong side effects for patients and their families

Inactive Publication Date: 2014-01-01
SHANGHAI PUBLIC HEALTH CLINICAL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, long-term interferon therapy has strong side effects and brings a heavy economic burden to patients and their families, while nucleoside analog therapy is prone to drug resistance of the virus

Method used

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  • Application of yeast-origin glucan on preparation of drugs for curing hepatitis B virus infection
  • Application of yeast-origin glucan on preparation of drugs for curing hepatitis B virus infection
  • Application of yeast-origin glucan on preparation of drugs for curing hepatitis B virus infection

Examples

Experimental program
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Embodiment 1

[0018] (1) Cell culture, clinical specimen intervention and index detection scheme

[0019] Isolate HBVC57BL / 6J-TgN transgenic mouse (the mouse was introduced from the Jackson laboratory of the United States, contains HBV S, PreS1 and PreS2 genes, and can express HBsAg in mouse serum) single splenocyte, DMEM culture medium (Gibco company, add 10% Fetal bovine serum, 100 U / ml penicillin, 100 μg / ml streptomycin and 400 μg / ml G418) in 5% CO 2 Culture at a constant temperature of 37°C in a saturated water vapor environment. Yeast-derived dextran was stimulated at 250 μg / ml for 48 hours, and the expression of cytokines was detected by quantitative PCR.

[0020] Isolate the bone marrow of HBVC57BL / 6J-TgN transgenic mice according to conventional methods, induce DC differentiation with 100ng / ml IL-4 and 100ng / ml GM-CSF for 8 days, add 10% fetal bovine serum, 100U / ml penicillin in DMEM culture medium (Gibco company) , 100 μg / ml streptomycin and 400 μg / ml G418) in 5% CO 2 Culture at...

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Abstract

The invention belongs to the field of medicine, relates to an application of yeast-origin glucan on preparation of drugs for curing hepatitis B virus infection, and especially relates to an application on preparation of drugs for reducing or eliminating serum surface antigen of chronic hepatitis B virus infected patients. It has been testified by tests that the yeast-origin glucan can modulate the cell factor expression of mice spleen cells, increases the expressions of IL-12, IL-1, and TNF, and decreases the expression of inhibiting cell factor IL-10; can promote the maturity of mDC of HBV C57BL / 6J-TgN transgenic mice, and increases the expressions of mDC surface co-stimulatory molecules CD80 and CD 86; is capable of prominently strengthening the generation of peripheral blood-derived PBMC IFN-gamma of HBV infected patients. The yeast-origin glucan can be used for preparation of drugs for curing hepatitis B infection, especially preparations of drugs for reducing or eliminating serum surface antigen of chronic hepatitis B virus infected patients, and can be taken as an immunity strengthening agent to help the treatment of HBV infection.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the new medicinal use of dextran, in particular to the application of yeast-derived glucan in the preparation of medicines for treating hepatitis B virus infection, especially in the preparation of serum for patients with chronic infection of hepatitis B virus that reduces or eliminates it Pharmaceutical applications of surface antigens. Background technique [0002] The prior art discloses that yeast-derived glucan (glucan) has a double-helical ultrastructure, and it has been combined with human monocytes, macrophages, neutrophil granulocytes, tree CR3, LacCer, Dectin-1, TLR2 and other receptors on the surface of immune cells such as dendritic cells (DC) and natural killer cells (NK cells) bind to play physiological functions such as recognition and phagocytosis, and stimulate the body to produce Antibodies secrete various cytokines such as TNF-α, interleukin and interferon, regulate the ...

Claims

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Application Information

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IPC IPC(8): A61K31/716A61P1/16A61P31/20A61P37/04
Inventor 袁正宏羽晓瑜周晓辉任广旭
Owner SHANGHAI PUBLIC HEALTH CLINICAL CENT
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