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Electrogenerated chemiluminescence immunosensor for detecting human interleukin 6 as well as preparation method and detection method of immunosensor

A technology of electrochemistry and luminescence immunity, which is applied in chemiluminescence/bioluminescence, scientific instruments, and analysis through chemical reactions of materials, etc., to achieve the effect of simple method, low detection cost and good stability

Active Publication Date: 2014-01-01
智德科技(无锡)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no electrochemiluminescent immunosensor based on graphene oxide nanosheet / polyaniline nanowire array / cadmium selenide quantum dot composite has been reported.

Method used

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  • Electrogenerated chemiluminescence immunosensor for detecting human interleukin 6 as well as preparation method and detection method of immunosensor
  • Electrogenerated chemiluminescence immunosensor for detecting human interleukin 6 as well as preparation method and detection method of immunosensor
  • Electrogenerated chemiluminescence immunosensor for detecting human interleukin 6 as well as preparation method and detection method of immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029]In this embodiment, the electrochemiluminescence immunosensor for detecting human interleukin-6 was prepared according to the following steps:

[0030] Step 1. Dissolve 50 μl of aniline in 20 ml of concentrated sulfuric acid solution with a concentration of 1 mol / L, and obtain a sulfuric acid solution of aniline after ultrasonic treatment for 0.5 h; add dropwise the described solution to 20 ml of graphene oxide solution with a concentration of 0.5 mg / ml After stirring the sulfuric acid solution of aniline for 30 minutes at room temperature, add 0.1512 g of ammonium persulfate, continue stirring for 12 hours, then centrifugally wash with deionized water until the pH is 6-7, and vacuum-dry at 65°C to obtain a dark green product, which is Graphene oxide nanosheet / polyaniline nanowire array composite, using deionized water as a solvent, the graphene oxide nanosheet / polyaniline nanowire array composite is formulated into a concentration of 0.5mg / ml graphene oxide nanosheet / pol...

Embodiment 2

[0039] In this example, an immunosensor was prepared by the same method as in Example 1 with EDC at a concentration of 30 mg / ml. The appearance and properties of the obtained immunosensor were similar to those of the immunosensor obtained in Example 1. The same immunoassay results were obtained.

Embodiment 3

[0041] In this example, an immunosensor was prepared by the same method as in Example 1 with a concentration of 20 mg / ml NHS. The appearance and properties of the obtained immunosensor were similar to those of the immunosensor obtained in Example 1. detection, the same immunoassay results were obtained.

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Abstract

The invention discloses an electrogenerated chemiluminescence immunosensor for detecting human interleukin 6. The electrogenerated chemiluminescence immunosensor is characterized in that the surface of a glassy carbon electrode is covered with a graphene oxide nanosheet / polyaniline nanowire array / cadmium selenide quantum dot composite film, the surface of the graphene oxide nanosheet / polyaniline nanowire array / cadmium selenide quantum dot composite film is treated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-Hydroxysuccinimide, and then a human interleukin 6 antibody is fixed on the surface of the graphene oxide nanosheet / polyaniline nanowire array / cadmium selenide quantum dot composite film. By means of the electrogenerated chemiluminescence immunosensor, human interleukin 6 is detected, the method is simple, the sensitivity is high, and the operation is easy.

Description

technical field [0001] The present invention relates to an electrochemiluminescent immunosensor for detecting human interleukin-6 and its preparation method and detection method, more specifically to a graphene oxide nanosheet / polyaniline nanowire array / cadmium selenide quantum dot (GO / PANI / CdSe) complex electrochemiluminescent immunosensor. Background technique [0002] Since British scientists discovered graphene in 2004, graphene, as a densely packed two-dimensional material of single-layer carbon atoms, has rapidly become a hotspot in the research of carbon materials. There are a large number of oxygen-containing groups on the surface of graphene oxide, which exhibits excellent electrical, thermal and mechanical properties, and has potential application value in the fields of microelectronic devices, liquid crystal devices and solar cells [see: (a) X.S.Li, Y.W. Zhu, W.W. Cai, M. Borysiak, B.Y. Han, D. Chen, R.D. Piner, L. Colombo, R.S. Ruoff, Nano Letters, 2009, 9, 4359...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/53G01N21/76
CPCG01N33/588G01N2800/7095
Inventor 毛昌杰胡晓炜石阳阳王程殷畅刘苹贞张胜义牛和林宋吉明
Owner 智德科技(无锡)有限公司
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