A kind of preparation method of compound strain in microbial fertilizer

A technology of microbial fertilizer and compound microbial inoculum, which is applied in the field of preparation of compound strains in microbial fertilizers, can solve the problems of drug residues, apple browning, apple discoloration, etc., maintain strain activity, prevent apple oxidation, and increase synergy effect of action

Active Publication Date: 2015-10-21
SHANDONG AIFUDI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, apples are one of the most popular and popular fruits in people's daily life. Apples are prone to discoloration, softening and rot, especially apple browning is the most common problem. Low temperature, air conditioning and chemical Preservatives are currently the most effective way to keep apples fresh, but there are common problems such as high cost and drug residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0024] A preparation method of composite strains in microbial fertilizer, 10 parts of polyacrylamide, 5 parts of matrine, 3 parts of ascorbic acid, 3 parts of EDTA, ZnCl 2 5 parts and 100 parts of microbial agent, and the rest are water.

[0025] The preparation steps are:

[0026] (1) Inoculate yeast, Bacillus licheniformis, Bacillus subtilis, photosynthetic bacteria, Streptomyces flavinus, and Bacillus lateralosporus into the primary culture medium respectively, and activate and cultivate them into primary strains; inoculate the primary strains respectively In the secondary medium, prepare the secondary single strain.

[0027] The primary culture medium of Streptomyces luteus is 1000 parts by weight of potato juice, 30 parts by weight of sucrose, 20 parts by weight of agar, the pH value is 7-8, and the temperature is 25-30 ° C; the secondary medium is 1000 parts by weight of potato juice, poly 1 weight part of acrylamide, 0.5 weight part of matrine, 0.5 weight part of asco...

Embodiment 2

[0036] A preparation method of composite strains in microbial fertilizer, 20 parts of polyacrylamide, 3 parts of matrine, 3 parts of ascorbic acid, 4 parts of EDTA, ZnCl 23 parts and 150 parts of microbial inoculants, and the rest are water.

[0037] (1) Inoculate yeast, Bacillus licheniformis, Bacillus subtilis, photosynthetic bacteria, Streptomyces flavinus, and Bacillus lateralosporus into the primary culture medium respectively, and activate and cultivate them into primary strains; inoculate the primary strains respectively In the secondary medium, prepare the secondary single strain.

[0038] The primary culture medium of Streptomyces luteus is 1000 parts by weight of potato juice, 50 parts by weight of sucrose, 15 parts by weight of agar, the pH value is 7-8, and the temperature is 25-30 ° C; the secondary medium is 1000 parts by weight of potato juice, poly 2 parts by weight of acrylamide, 0.3 parts by weight of matrine, 0.3 parts by weight of ascorbic acid, 0.4 parts ...

Embodiment 3

[0047] A preparation method of composite strains in microbial fertilizer, 15 parts of polyacrylamide, 4 parts of matrine, 3 parts of ascorbic acid, 3 parts of EDTA, ZnCl 2 4 parts and 50 parts of microbial inoculants, and the rest are water.

[0048] (1) Inoculate yeast, Bacillus licheniformis, Bacillus subtilis, photosynthetic bacteria, Streptomyces flavinus, and Bacillus lateralosporus into the primary culture medium respectively, and activate and cultivate them into primary strains; inoculate the primary strains respectively In the secondary medium, prepare the secondary single strain.

[0049] The primary medium of Streptomyces luteus is 1000 parts by weight of potato juice, 20 parts by weight of sucrose, 25 parts by weight of agar, the pH value is 7-8, and the temperature is 25-30 ° C; the secondary medium is 1000 parts by weight of potato juice, poly 1.5 parts by weight of acrylamide, 0.4 parts by weight of matrine, 0.3 parts by weight of ascorbic acid, 0.3 parts by wei...

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PUM

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Abstract

The invention discloses a method for preparing compound strains in microbial fertilizers. The strains in the compound microbial strains are separately fermented to obtain single strains of each strain; then the single strains are mixed and fermented to obtain composite microorganisms Bacteria. The compound microbial fertilizer prepared by the method can effectively maintain the activity of strains, has strong synergy between strains and high fertilizer efficiency.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a method for preparing composite strains in microbial fertilizers. Background technique [0002] There are a variety of microbial organic fertilizers on the market now, and today, as people are increasingly pursuing a higher quality of life, the development of efficient and safe fertilizers has become a development trend. Most of the existing fruit tree fertilizers can cause drug residues, many of which have strong toxic and side effects on the human body, and the cost is high and the production process is complicated. Due to the excessive use of chemical fertilizers and the continuous planting of crops, the soil began to compact in a large area, the beneficial bacteria in the soil became less and less, and soil-borne diseases such as heavy cropping and root-knot nematodes became more and more serious; Streaks, poor disease resistance, frequent occurrence of variou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C12N1/14C12N1/20C12R1/10C12R1/125C12R1/465
Inventor 魏祥圣
Owner SHANDONG AIFUDI BIOLOGICAL TECH
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