A method for producing cellulase by fermenting corncobs as main raw material

A technology of the main raw material, cellulase, which is applied in the field of producing cellulase by fermenting corncobs as the main raw material, which can solve the problems of high cost and achieve the effects of low cost, low production and preparation costs, and low price

Active Publication Date: 2018-01-09
湖南康捷生物科技有限公司
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Problems solved by technology

[0003] However, the research on the preparation of cellulase so far has shown that the cost of raw materials for the production of cellulase is relatively high, and the source of corncobs in rural areas is extensive, which prompts us to explore and study the method of turning waste into treasure and preparing cellulase at low cost. The present invention is exactly under such guiding ideology, obtains success through numerous tests

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  • A method for producing cellulase by fermenting corncobs as main raw material

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Embodiment 1

[0043] On a sterile ultra-clean bench, take a slant strain, add 10 ml of 0.9% physiological saline, scrape off the spores on the culture medium with a bamboo stick, and obtain the strain spore liquid. Get 1ml of bacterial seed spore liquid and put it into a 250ml triangle containing 50ml of seed medium, and place it on a shaker. Culture conditions: rotation speed 200rpm, temperature 28°C, constant temperature cultivation for 50 hours, which is the seed liquid for fermentation and enzyme production.

[0044] Corncobs are prepared into corncob powder through pulverization, according to corncob 20g / L, corn steep liquor 12g / L, (NH4) 2 SO 4 1.2g / L, KH 2 PO 4 2g / L, MgSO 4 7H 2 O0.2g / L, CaCl 2 Mix 5 liters of enzyme-producing medium at a ratio of 0.2g / L, adjust the pH to 5.0 with phosphate, put it into a 7-liter fermenter, add 2ml of antifoaming agent, and sterilize at 121°C for 30 minutes.

[0045] The cultivated seeds are inoculated into a 7-liter fermenter equipped with 5 l...

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Abstract

The present invention provides a method for producing cellulase through fermentation by adopting corn cob as a main raw material, wherein the adopted fermentation strain is preserved in the China Center for Type Culture Collection on June 5, 2012, has a preservation number of CCTCC M 2012204, and is the Trichodema reesei QZ-5 strain. The method is characterized in that agricultural product waste corn cob is adopted as a main raw material to carry out fermentation production to prepare cellulase, the international standard method recommended by the International Union of Pure and Applied Chemistry (IUPAC) is adopted to determine enzyme activity (ie., filter paper enzyme activity FPA determination), the highest enzyme activity of the cellulase prepared through fermentation can be more than 8 IU / ml, the cost is low, the waste is changed into treasure, the method is simple and easy to perform, and the produced cellulase can be widely used in fields of textiles, papermaking, feeds, food and the like, and belongs to the technical field of biology.

Description

technical field [0001] The fermentation strain that the present invention adopts is the Trichoderma reesei QZ-5 cellulase-producing Trichoderma reesei Trichoderma reesei strain that was preserved in the China Type Culture Collection Center on June 5, 2012. The preservation number is: CCTCC M 2012204 , the feature of this invention is to ferment and produce cellulase from agricultural product waste corncob as the main raw material, and use the filter paper enzyme activity FPA method to measure the enzyme activity, the highest enzyme activity can reach more than 8IU / ml, the cost is low and the waste can be converted into Treasure, easy to do. The produced cellulase can be widely used in textile, papermaking, feed, food and other fields, and belongs to the field of biotechnology. Background technique [0002] Cellulose is the most widely distributed, most abundant, and cheapest natural renewable resource in nature, accounting for more than 50% of the carbon content in the plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/885
CPCC12N9/2437
Inventor 张峰李洪兵张明
Owner 湖南康捷生物科技有限公司
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