Joint detection kit for sialic acid and hydroxyproline

A hydroxyproline and combined detection technology, applied in the field of medical testing, can solve the problems of expensive instruments, low sensitivity, lack of combined detection, etc., and achieves the effects of simple operation, easy popularization and application, and improved sensitivity

Active Publication Date: 2014-01-15
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chloramine T colorimetric method is a traditional method for the determination of hydroxyproline, but this method has poor specificity and low sensitivity, and has not been widely used at present.
The detection of hydroxyproline by high performance liquid chromatography and automatic amino acid analyzer also has the problems of expensive equipment and inconvenient detection, which limits the clinical batch application
[0005] It can be seen that although there are many detection methods for sialic acid and hydroxyproline at present, they all have various problems and cannot be routinely used in clinical practice, and there is even a lack of combined detection methods for the two. , low cost, convenient and applicable combined detection method of sialic acid and hydroxyproline is of great significance for improving the sensitivity and specificity of tumor diagnosis

Method used

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  • Joint detection kit for sialic acid and hydroxyproline
  • Joint detection kit for sialic acid and hydroxyproline
  • Joint detection kit for sialic acid and hydroxyproline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A combined detection kit for sialic acid and hydroxyproline, consisting of the following two reagents, wherein the concentration of each component in the chromogenic reagent is:

[0035]

[0036] The reference product liquid is composed of sialic acid, hydroxyproline and water, and the concentration of each component is as follows:

[0037] Sialic acid 0.3g / L

[0038] Hydroxyproline 0.25g / L.

[0039] The preparation method of the color developer and the reference product solution is a conventional method, that is, the components described in the color developer and the reference product are respectively added to distilled water and then mixed and stirred evenly.

[0040] The method for determining sialic acid and hydroxyproline using the above kit is as follows:

[0041] (1) In each reaction, make three parallel tubes for the reference quality control, and one tube for the test tube sample.

[0042] (2) Operate according to the procedures in Table 1; mix well, pla...

example 1

[0049] Example 1, kit and sialic acid, hydroxyproline reactivity experiment

[0050] The kit prepared in Example 1 detects sialic acid, hydroxyproline, sialic acid+hydroxyproline, and the curve of concentration and detection value is as follows: figure 1 , figure 2 , image 3 Shown (absorbance at 450nm). The absorption peak curve is as Figure 4 shown.

[0051] Depend on Figure 4 It can be seen that the reaction of sialic acid with this kit produces a colorimetric maximum absorption peak at 450nm, and is determined by figure 1 It can be seen that the detection value changes linearly with the increase of concentration.

[0052] Depend on Figure 4 It can be seen that the reaction of hydroxyproline with this kit produces the colorimetric maximum absorption peak at 450nm, and is determined by figure 2 It can be seen that the detection value changes linearly with the increase of concentration.

[0053] We mixed sialic acid and hydroxyproline to prepare a mixed concentr...

example 2

[0061] Example 2, kit stability experiment

[0062] The detection reagent was opened, and the stability performance of the reagent during use was tested. It was found that the result remained stable within 60 days after opening the bottle, as shown in Table 4.

[0063] Table 4 Kit Stability Experiment

[0064]

[0065]

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PUM

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Abstract

The invention discloses a joint detection kit for sialic acid and hydroxyproline. The kit comprises a color developing agent and a reference solution. The color developing agent is made from diethyl phthalate, ethyl acetate, 2,4-dinitrophenylhydrazine, hydrogen peroxide, hydrochloric acid and water, wherein the concentrations of the components are as follows: 0.35 mol/L of diethyl phthalate, 0.46 mol/L of ethyl acetate, 0.07 mol/L of 2,4-dinitrophenylhydrazine, 0.006 mol/L of hydrogen peroxide, and 0.62 mol/L of hydrochloric acid (hydrogen chloride). The reference solution is made from sialic acid, L-hydroxyproline and water, wherein the concentrations of the components are as follows: 0.3 g/L of sialic acid, and 0.25 g/L of L-hydroxyproline. With a specific color-developing analysis technology, in the same detection system, through primary reaction, multiple tumor markers can be reacted to develop colors. As the developed colors are superposed, the sensitivity of detection is improved. The joint detection kit complies with the trend of joint detection for tumor markers, is convenient and simple to operate, and is easy to promote and apply since the kit is operated without valuable equipment.

Description

technical field [0001] The invention relates to a combined detection kit for sialic acid and hydroxyproline, which belongs to the technical field of medical testing. Background technique [0002] Sialic acid, also known as N-acetylneuraminic acid, is a natural neuraminic acid derivative. Its parent structure is an endocyclic amino acid composed of nine carbon atoms. It is a general term for a family of compounds. Sialic acid is widely distributed in animals. Sialic acid in human serum includes free sialic acid and conjugated sialic acid. The content of the former is relatively low. Conjugated sialic acid bound to lipoproteins and glycoproteins is the main form of sialic acid in serum. Hydroxyproline, an imino acid, is the product of hydroxylation of proline, usually with a hydroxyl group at the 4th position, but sometimes at the 3rd position. Since there are two asymmetric carbon atoms, there are 4 stereoisomers. L-hydroxyproline exists naturally in nature, and D-hydroxypr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 王传新张义张欣杜鲁涛李培龙
Owner SHANDONG UNIV QILU HOSPITAL
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