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Application of mango extracts to preparation of anti-tumor drugs

A technology of extract and mango, which is applied in the field of medicine, can solve the problem that the effect of inhibiting tumors has not been reported.

Active Publication Date: 2014-01-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The by-products of mango processing, the peel and seed kernels, contain a large amount of gallic tannins, and there are relatively few reports on their pharmacological activities, most of which focus on anti-oxidation and antibacterial activities, but their anti-tumor effects have not been seen. to report

Method used

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  • Application of mango extracts to preparation of anti-tumor drugs
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  • Application of mango extracts to preparation of anti-tumor drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1 The operation method of extracting and separating the gallic tannin extract of the present invention is carried out according to the following two steps:

[0021] (1) Enrichment of gallic tannins from mango by-products: 1.5 g of Marchesu (MQS), Tainong No. 1 (TN1), Zihuamang (ZHM) fruit kernels and peel freeze-dried powder were weighed respectively, and 80% Acetone (solid-to-liquid ratio 1:20) ultrasonically extracted twice for 30 minutes, centrifuged at 8000 rpm for 10 minutes, combined the two supernatants, evaporated to dryness on a rotary evaporator to obtain extract, dissolved in 25 ml ddH 2 O middle.

[0022] (2) C18 solid-phase extraction column (Waters 12cc, 2 g) was first activated with 15 ml of methanol, then equilibrated with 15 ml of water, and then loaded with 25 ml of the extract, after fully adsorbed, rinsed with 25 ml of water to remove Water-soluble impurities such as sugar and acid were then eluted with 25 ml of methanol, and impur...

Embodiment 2

[0024] Mango gallotannin extract significantly inhibited cancer cell proliferation after acting on human breast cancer cells (MDA-MB-231 cells) for 72 h. The specific steps are as follows: Digest the cells in the logarithmic growth phase, pipette into a single cell suspension, and inoculate in a 96-well culture plate; 5 x 10 3 Cells / well, 200 microliters of culture medium per well, cultivate overnight in an incubator at 37° C. with 5% CO2; add different concentrations of mango gallotannin extracts and culture in the incubator for another 3 days. Fix with 10% trichloroacetic acid for 1 hour, wash with double distilled water, and dry, add 70 microliters of SRB solution (4 mg / mL) to each well, stain at room temperature for 20 minutes, wash with 1% acetic acid, and dry. Add 100 μl of 10 mM Tris-Base solution to each well to dissolve SRB. Detect the OD value of each well with a microplate reader (detection wavelength: 515 nm); record the results; calculate the inhibition rate acco...

Embodiment 3

[0026] Mango gallic tannin extract significantly inhibited the proliferation of cancer cells after acting on HepG2 cells for 72 h. The specific steps are as follows, see Example 2, the IC50 of the 3 varieties of mango kernel and peel extracts are 33.91 ± 3.06, 41.05 ± 9.99, 40.43 ± 8.33, 43.01 ± 7.59, 43.30 ± 15.49, 53.33 ± 1.81 μg / mL, the specific concentration The inhibitory effect of the extract on HepG2 cells is shown in image 3 .

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Abstract

The invention provides an application of mango extracts to preparation of drugs or health care products for controlling tumor diseases. The mango extracts are crude pure powder obtained by extracting mango peels or kernels with 80% of acetone and carrying out primary purification on the extracts through C18 solid phase extraction. The main ingredients of the mango extracts include fivefold gallotannin and derivatives thereof. The drugs or the health care products are prepared from the mango extracts and preparation allowable excipients or food additives. Researches prove that the mango extracts have the activity of inhibiting breast cancers, liver cancers and leukemia and can be used for preparing the drugs for treating breast cancers, liver cancers and leukemia and inhibiting cell activities of breast cancers, liver cancers and leukemia or can be developed into functional food or health care products. The invention provides a new application of mango processing byproducts, namely the mango peel and kernel extracts to tumor treatment drugs or health care products.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of mango extracts from natural sources in the prevention and treatment of breast cancer, liver cancer, leukemia and other related cancers, in particular to the application of mango gall tannin extracts in the preparation of functional foods or health products for the prevention and treatment of cancer. Background technique [0002] With the changes of many natural and social factors such as human living environment, living pressure, population aging, etc., the incidence and mortality of malignant tumors continue to rise, seriously threatening human health and life. The 2012 China Cancer Registration Annual Report shows that there are approximately 3.12 million new cancer cases each year, 6 people are diagnosed with malignant tumors every minute in the country, and the national cancer mortality rate is 180.54 / 100,000, 1 in every 7 to 8 people is due to Malignant tumor death. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/22A23L1/29A23L1/212A61P35/00A61P35/02A23L19/00A23L33/00
Inventor 朱虹陈昆松何俏军杨波李鲜孙崇德
Owner ZHEJIANG UNIV
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