A kind of crab meat flavor and preparation method thereof
A flavoring material and crab meat technology, which is applied in food preparation, food ingredients containing natural extracts, food science, etc., can solve problems affecting the taste of crab meat and crab-flavored products, and achieve low cost and easy factory production , The effect of overcoming technical barriers
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Embodiment 1
[0016] Example 1 Preparation of Crabmeat Water Extract
[0017] Take 10g of freshwater crab (such as Chinese mitten crab) crab meat, add about 30g of deionized water, homogenize for 0.5min, and repeat 5 times. Then in a vacuum refrigerated centrifuge, centrifuge at 4°C for 5 minutes, and the supernatant is the crab meat aqueous extract.
Embodiment 2
[0018] Embodiment 2 Chromatographic separation of water extract
[0019] Chromatography device: consists of peristaltic pump, ultraviolet detector, chromatography column (40cm×1cm), filler (Sephadex G-25, the volume after water activation is 40mL), chromatography eluent (deionized water ), the collector, and the operating temperature is 4°C.
[0020] Take 1 mL of the crab meat water extract prepared in Example 1 and add it to the chromatography column, start the peristaltic pump, the eluent flow rate is 1 mL / min, the collector speed is 3 minutes / tube, and the elution time is 2.5 hours. After the eluate passes through the ultraviolet detector, the electric signal is displayed on the printing paper, and the detection wavelength is 220nm.
[0021] According to the strength of the electrical signal, there are three ultraviolet absorption peaks, such as figure 1 shown.
Embodiment 3
[0022] Example 3 Freeze-drying
[0023] Collect the chromatographic elution fractions from the 21st to 42nd minutes, the 42nd to 72nd minutes, and the 81st to 132nd minutes in Example 2 respectively, and after freeze-drying, three components F-i, F-ii and F-iii are obtained . The recovery rate of F-i was 59%, and that of F-ii was 33%.
[0024] The freeze-drying condition is -40°C, and the time is 24 hours / 10mL sample.
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