Method for building chronic myocardial ischemia animal model

A technology of myocardial ischemia and animal models, which is applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problem that it is difficult to determine the ischemia site, cannot ideally reproduce myocardial ischemia, and cannot diagnose and treat myocardial ischemia. and research issues to achieve the exact effect of ischemia

Inactive Publication Date: 2014-02-26
王景文
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Problems solved by technology

However, it is difficult to determine the ischemic site in the laboratory at present, so the process of myocardial ischemic i

Method used

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Embodiment Construction

[0006] A method for constructing an animal model of long-term chronic myocardial ischemia, characterized in that first, a sterile surgical kit and a small animal ventilator are prepared; the main process of model establishment: the rat is weighed, anesthetized, subcutaneously injected with atropine, and the rat is placed in a supine position The position was fixed on a self-made wooden platform; the neck and chest surgical site were sheared, and iodophor was disinfected; ECG monitoring was performed on the rat; hole towels were spread, the tracheotomy was intubated, and a ventilator was connected to assist breathing; After each layer of tissue, enter the chest cavity to find the beating heart; use two small retractors to expose the heart; use tweezers to tear the pericardium, gently push the left atrial appendage with cotton sticks, and expose the root of the aorta, between the left atrial appendage and the pulmonary artery The blood vessels were ligated at the lower edge of th...

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Abstract

The invention discloses a method for building a chronic myocardial ischemia animal model. The method comprises the steps of preparation of sterile operation kit and a small animal respirator; main processes of model building: a mouse is weighed, anesthetized, subjected to atropine hypodermic injection and fixed on a self-made plank platform in a supine position; the neck and chest operation sites are sheared and sterilized through iodophor. The method has the advantages that no mouse is infected through the serious sterile operation in the experiment. The ischemia model is built, ischemia is clear, the myocardial ischemic injury process can be duplicated ideally, and the further study on ischemic heart failure animals, in particular to the study on physiopathologic mechanisms, such as myocardial remodeling after ischemia, is facilitated.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing an animal model of long-term chronic myocardial ischemia. Background technique [0002] Myocardial ischemia, injury, infarction, and finally heart failure, referred to as heart failure, is the most common disease in contemporary society, so the study of its pathophysiological mechanism is particularly important, and the successful establishment of animal models can provide a basis for its research. Ligation of coronary arteries in rats is a classic and widely adopted method. However, it is difficult to determine the ischemic site in the laboratory at present, so the process of myocardial ischemic injury cannot be ideally replicated, and the diagnosis, treatment and research of myocardial ischemia cannot be performed well. Contents of the invention [0003] To overcome the above technical problems, we propose the following technical solution...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
Inventor 王景文
Owner 王景文
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