Method for building long-term chronic myocardial ischemia rat model

A technology of myocardial ischemia and rat model, applied in the fields of medical science, veterinary equipment, veterinary surgery, etc., can solve the problem that it is difficult to determine the ischemia site, cannot diagnose, treat and study myocardial ischemia, and cannot ideally replicate Myocardial ischemia and other problems, to achieve the exact effect of ischemia

Inactive Publication Date: 2014-02-26
刘军
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AI Technical Summary

Problems solved by technology

However, it is difficult to determine the ischemic site in the laboratory at present, so the process of myocardial ischemic injury cannot be ideally replicated, and the diagnosis, treatment and research of myocardial ischemia cannot be performed well.

Method used

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Examples

Experimental program
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Embodiment Construction

[0008] Anesthesia: use fast metabolism and small dose of anesthetic; when using the ventilator, the tidal volume used is 3.0ml, 70 times / min, when intubating: cut the intubation first, use the plastic tube at the front of the scalp needle, the incision should be sufficient Large; determination of blood vessels: choose between the left atrial appendage and the conus pulmonary artery for ligation; maintain the anatomical structure; monitor the state of the rat after thorax closure; postoperative pathological tissue sections to clarify the effect of ligation.

[0009] The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Anyone familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention Any equivalent replacement or change of the inventive concepts thereof shall fall within the protection scope o...

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Abstract

The invention discloses a method for building a long-term chronic myocardial ischemia rat model. The method comprises the steps of anesthesia: a small dose of fast-metabolism anesthetic is selected; using a respirator: wherein the use tidal volume is between 2.0 and 4.0ml for 70 times / min; cannula implantation: wherein a cannula is cut open, a plastic tube at the front end of a scalp needle is used, and the incision is required to be big enough; vessel determination: ligation is performed to select the vessel between the left aurcle and the pulmonary artery cone; maintaining of the anatomical structure; monitoring of the mouse state after chest shutting; determining of the ligation effect through the histopathologic slide after the surgery. The method has the advantages that no mouse is infected through the serious sterile operation in the experiment. The ischemia model is built, ischemia is clear, the myocardial ischemic injury process can be duplicated ideally, and the further study on ischemic heart failure animals, in particular to the study on physiopathologic mechanisms, such as myocardial remodeling after ischemia, is facilitated.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing a long-term chronic myocardial ischemia rat model. Background technique [0002] Myocardial ischemia, injury, infarction, and finally heart failure, referred to as heart failure, is the most common disease in contemporary society, so the study of its pathophysiological mechanism is particularly important, and the successful establishment of animal models can provide a basis for its research. Ligation of coronary arteries in rats is a classic and widely adopted method. However, it is difficult to determine the ischemic site in the laboratory at present, so the process of myocardial ischemic injury cannot be ideally replicated, and the diagnosis, treatment and research of myocardial ischemia cannot be performed well. Contents of the invention [0003] In order to overcome the above technical problems, we propose the following technical soluti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00
Inventor 刘军
Owner 刘军
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