Application of hydrogen sulfide and donor thereof sodium hydrosulfide to preparation of medicament for treating diabetes
A diabetes drug, sodium hydrosulfide technology, applied in the direction of drug combination, urinary system diseases, metabolic diseases, etc., can solve problems such as adverse side effects, inappropriate use, etc., to increase insulin levels, reduce oxidative stress, and prevent diabetic kidney disease Sign effect
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Examples
Embodiment 1
[0033] Example 1 Hydrogen sulfide and its donor sodium hydrosulfide as insulin sensitizer test
[0034] After incubation with different concentrations of NaHS (1-200 μmol / L) for 24 hours, it has no effect on the glucose uptake of normal skeletal muscle cells in the basal state. It was tested whether NaHS has an effect on the glucose uptake of skeletal muscle cells in the presence of insulin. Detect the effects of different concentrations of NaHS (0-200μmol / L) on glucose uptake under insulin stimulation after incubating glucose (5.5μmol / L) and insulin (100nmol / L) resistant skeletal muscle cells for 24 hours, the results show (eg figure 1 shown), the concentration of NaHS increased significantly at 25, 50 and 100 μmol / L 3 H-deoxyglucose uptake, the optimal concentration of NaHS to promote glucose uptake is 100 μmol / L, which is more than doubled compared with the control group, 2.06±0.29 (P<0.01), 25 and 50 μmol / L NaHS respectively increase glucose uptake The ratios are 1.54±0.0...
Embodiment 2
[0050] Example 2 Correlation test between skeletal muscle and fat cells and insulin resistance
[0051] In this embodiment, insulin resistance and diabetes cells widely used in the prior art are used: wherein, the skeletal muscle myoblast cell line is isolated from the primary culture of rat thigh muscle by Yaffe, and can be obtained under certain culture conditions. Fusion forms multinucleated myotubes and striated muscle fibers; adipocyte lines are derived from mouse embryonic fibroblasts, which are cloned and expanded into preadipocyte cell lines, and then induced to differentiate into mature adipocytes by specific differentiation agents;
[0052] 1) Evaluation of insulin sensitivity at the cellular level
[0053] Using isotope-labeled glucose uptake experiments by cells to evaluate the ability of cells to uptake and utilize glucose stimulated by insulin, thereby evaluating insulin sensitivity and judging whether insulin resistance exists;
[0054] Glucose consumption test...
Embodiment 3
[0061] Example 3 Hypoglycemic and insulin-sensitizing effects of hydrogen sulfide and its donor sodium hydrosulfide on type Ⅱ diabetes
[0062] Select 2-month-old GK rats as the animal model of diabetic insulin resistance in this embodiment;
[0063] Insulin tolerance test was routinely carried out, and the experimental results showed that 60 minutes after the chronically administered rats were injected with a certain amount of insulin, the ratio of blood glucose drop in the NaHS 30 μmol / kg group of GK rats was significantly greater than that of the control group (such as Figure 9 shown), the ratio of blood glucose drop in the Wistar rat NaHS 60 μmol / kg group was significantly greater than that of the control group (such as Figure 10 shown); Insulin sensitivity test, using low concentration of insulin (0.4U / kg) to starve Wistar rats for 4 hours, the NaHS 60μmol / kg group was significantly lower than the control group (such as Figure 11 shown), the results show that a small ...
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Application Information
- IPC
- A61K33/04; A61P3/10; A61P5/50; A61P13/12
- Inventors
- 朱依纯; 薛蓉