Method for preparing mannooligosaccharide from konjac powder

Abstract

The invention provides a method for preparing mannooligosaccharide from konjac powder. The mannooligosaccharide is obtained by utilizing the synergistic effect of recombinant beta-mannanase (reAuMan5AN3C3) and recombinant beta-endoglucanase (reAuMan5AN3C3) from a laboratory to perform enzymolysis on the konjac powder to obtain mannooligosaccharide. The catalytic efficiency of a key enzyme used by the method is relatively high, the production and using cost of the enzyme is relatively low, the period is short, the pollution is avoided, and the method has relatively great industrial production and application potential and economic values.

Description

technical field [0001] The invention relates to a method for preparing mannose oligosaccharides by hydrolyzing konjak flour with biological enzymes, and belongs to the technical field of bioengineering. Background technique [0002] Mannooligosaccharide (Mannooligosaccharide, MOS) is an oligosaccharide polymerized by 2 to 10 mannose through β-1,4 glycosidic bonds, also known as mannooligosaccharide. It can effectively promote the proliferation of intestinal probiotics represented by bifidobacteria in organisms, and has the functions of inhibiting the growth of pathogenic bacteria in the body, reducing the production of toxic metabolites, preventing constipation, protecting the liver, anti-tumor and enhancing the body's immunity, etc. Physiological function, is a new generation of functional food. my country has formulated functional oligosaccharide industry standards and listed mannose oligosaccharides as one of them, but the market share of corresponding domestic products ...

AI Technical Summary

Problems solved by technology

At present, mannose oligosaccharides are usually prepared by high-temperature hydrolysis, acid hydrolysis or alkaline hydrolysis. Although enzymatic hydrolysis has also been reported, the conditions are not mature enough.

The enzymatic production of mannose oligosaccharides is mainly limited by the low catalytic efficiency of the key enzymes used in the production, the high cost of enzyme production and use, and the high viscosity of the substrate, which makes it difficult to achieve high-concentration production, etc.