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Method and system for detecting drug resistance of beta-lactam antibiotics and application of beta-lactam antibiotics

A technology for antibiotic resistance and lactams, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection accuracy and long detection time, and achieve high sensitivity, short detection time and high selectivity Effect

Active Publication Date: 2014-03-19
宁波诺威科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Both the above-mentioned disc diffusion method and turbidimetric method have the following problems: the detection process is affected by various factors such as microbial concentration, purity and operator's skill level, the detection accuracy is low, and the detection time is long

Method used

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  • Method and system for detecting drug resistance of beta-lactam antibiotics and application of beta-lactam antibiotics
  • Method and system for detecting drug resistance of beta-lactam antibiotics and application of beta-lactam antibiotics
  • Method and system for detecting drug resistance of beta-lactam antibiotics and application of beta-lactam antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] Embodiment 1, the detection of Escherichia coli drug resistance to cefotaxime

[0123] The reference molecular weight of cefotaxime is 455.46, and its structural formula is as follows:

[0124]

[0125] 1. Preparation of Escherichia coli suspension

[0126]After the clinically infected blood was diluted, it was inoculated on a common nutrient agar culture plate, and after culturing the isolated Escherichia coli, the pure cultured colonies were picked and suspended in normal saline to make a suspension of Escherichia coli with 0.6 McFarland units.

[0127] 2. Prepare the solution to be tested

[0128] The embodiment of the present invention carried out 6 experiments, and specifically prepared the mixed solution according to the reagents shown in Table 1:

[0129] The preparation proportion of reagent in the mixed solution of table 1

[0130]

[0131] Reagents were added to 6 clean test tubes according to Table 1, and after standing for 20 minutes, buffer solutio...

Embodiment 2

[0146] Embodiment 2, the detection of Escherichia coli resistance to ceftazidime

[0147] The reference molecular weight of ceftazidime is 546.58, and its structural formula is as follows:

[0148]

[0149] 1. Preparation of E. coli suspension

[0150] After the clinically infected blood was diluted, it was inoculated on a common nutrient agar culture plate, and after culturing the isolated Escherichia coli, the pure cultured colonies were picked and suspended in normal saline to make a suspension of Escherichia coli with 0.6 McFarland units.

[0151] 2. Preparation of test samples

[0152] The embodiment of the present invention carries out 6 experiments, specifically prepares the mixed solution according to the reagent shown in table 3:

[0153] The preparation proportion of reagent in the mixed solution of table 3

[0154]

[0155] Reagents were added to 6 clean test tubes according to Table 2, and after standing for 30 minutes, buffer solution was added to each te...

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Abstract

The invention relates to the filed of drug detection, and particularly relates to a method and system for detecting drug resistance of beta-lactam antibiotics and application of beta-lactam antibiotics. The method comprises steps of mixing turbid liquid of microorganism to be tested with standard liquid of beta-lactam antibiotics, then removing the microorganism to be tested so as to obtain the liquid to be tested; obtaining liquid chromatogram of the liquid to be tested; judging the tolerance of the microorganism to be tested to the beta-lactam antibiotic according to the liquid chromatogram. The detection is not affected by concentration and purity of the microorganism and operation technique of an operator, so that the method has the advantages of high sensitivity, high accuracy, high detection speed and short detection time, and can be implemented within two hours. The detection method uses fewer microorganisms, does not need subculture for many times, greatly shortens the time of the whole experiment, provides prompt basis for rapid and early clinical drug use, especially for seriously infected patients; and since the detection time is greatly shortened, the consumable cost is low.

Description

technical field [0001] The invention relates to the field of drug detection, in particular to a detection method, system and application of β-lactam antibiotic resistance. Background technique [0002] β-lactam antibiotics include penicillin and its derivatives, cephalosporins, monoamide rings, carbapenems and penem enzyme inhibitors, etc., due to their strong bactericidal activity, low toxicity, and wide indications And the advantages of good clinical curative effect have been widely used in clinical practice. [0003] Drug resistance (Resistance to Drug), also known as drug resistance, refers to the tolerance of microorganisms, parasites, and tumor cells to the effects of chemotherapy drugs. Once drug resistance occurs, the chemotherapy effects of drugs will be significantly reduced. [0004] The mechanism of microbial resistance to antibiotics includes: (1) changes in cell membrane permeability, so that antibiotics cannot, or rarely penetrate into microorganisms to reach...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/72
Inventor 佟雪梅赵焕铸
Owner 宁波诺威科技有限公司
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