Device and method for quick extraction of electrical stimulation earthworm body fluid

Abstract

The invention relates to a device and a method for quick extraction of electrical stimulation earthworm body fluid, and aims to solve the technical difficult problems that the natural secretion of earthworm body fluid is little and the earthworm body fluid is difficult to collect on the premise of keeping the components and nature of the earthworm body fluid unchanged and trying to reducing the injury to the living earthworm, and meet the quick extraction requirement of earthworm body fluid in experimental investigation. The device comprises an extraction box, a separation plate and a collection box which adopts ultra-high molecular weight polyethylene and other hydrophobic material; the hole pattern of the separation plate is a conical counter bore; one end of each metal electrode plate is provided with an insulated handle, and each conductive end and the corresponding insulated handle are placed adjacently to form a pole plate group with an adjustable distance; the output voltage range of an adjustable direct current power supply is adjustable. The method comprises the steps that the cleaned living earthworm washed is arranged among the pole plate groups, spacing between adjacent pole plates is adjusted to tightly clamp the earthworm; after the power supply is electrified, the earthworm body is communicated with every two pole plates to form loop current, and the earthworm is stimulated to secrete a large amount of body fluid; the body fluid drops into the collection box through the separation plate under the action of gravity, and the crude extract of earthworm body fluid is obtained.

Description

technical field

[0001] The invention relates to a device and method for extracting biological body fluids, in particular to a device for rapidly extracting earthworm body fluids by means of electrical stimulation, separating and collecting the body fluids in time, and a matching operation method. Background technique

[0002] Earthworm body fluid is an important part of earthworm respiratory system and immune defense system. Earthworm body fluid is formed by mixing surface fluid and body cavity fluid. Earthworm body surface fluid is a colorless slightly viscous liquid secreted by mucus cells, which can prevent earthworm skin from drying out, dissolve oxygen for earthworm respiration, and excrete N in the body in the form of mucus protein. As an immune barrier, earthworm coelom fluid contains various types of leukocytes and hemolytic proteins, peptides and other biologically active molecules, which have the biological characteristics of selectively inhibiting bacteria and ly...

Examples

Embodiment 1

[0040] The device and method for rapid extraction of electric stimulation type earthworm body fluid in this example refer to Figure 1-Figure 5, Figure 6a1-2. The characteristics of the device in this example are: the upper part of the extraction device is an extraction box, the side of which is equipped with terminal posts, connected to the embedded metal guide rail, the bottom surface is a separation plate, the hole structure adopts the form of a conical counterbore, the hole diameter d is 3mm, and the depth h is 3mm, chamfer angle θ is 20°, hole spacing x and y are both 10mm; 4 L-shaped electrode plates are placed on the metal guide rail, and the metal conductive end and the insulating handle of the adjacent electrode plate are adjacent on the same metal guide rail Placed to form 3 sets of L-shaped plates with adjustable distances. After power-on, the 4 L-shaped plates will show alternating positive and negative voltages; the lower part is the collection box, and the side i...

Embodiment 2

[0042] The device and method for rapid extraction of earthworm body fluid in this example refer to Figure 4 , Figure 5 , Figure 6b1-2. The device described in this example is different from Example 1 in that: the diameter d of the conical counterbore of the separation plate of the extraction device is 4mm, the depth h is 4mm, the chamfer angle θ is 30°, and the hole spacing x and y are 15mm; 4 The T-shaped electrode plates are placed on the metal guide rails to form 3 sets of T-shaped electrode plates with adjustable distances. The method described in this example is different from Example 1 in that: Take 300 g of sexually mature trapezoidal worms (Aporrectodea trapezoides Dugès, 1828) and wash them with clean water, then place them between three groups of plates respectively, and adjust the distance between the plates a, Both b and c are 10mm, and the earthworms are tightly clamped; the 9V output is selected, and the stimulation time is controlled to 45s. The rest are th...

Embodiment 3

[0044] The device and method for rapid extraction of earthworm body fluid in this example refer to Figure 4 , Figure 5 , Figure 6c1-2. The difference between the device described in this example and Embodiment 1 and Embodiment 2 is that the diameter d of the conical counterbore of the separation plate of the extraction device is 5mm, the depth h is 5mm, the chamfer angle θ is 45°, and the hole spacing x and y are both 20mm; 4 E-type electrode plates are placed on the metal guide rail to form 3 sets of E-type electrode plates with adjustable distance. The method described in this example is different from Example 1 and Example 2 in that: take 500 g of sexually mature Amynthas pingi Stephenson (Amynthas pingi Stephenson, 1925) and wash them with clean water and place them between 3 groups of plates respectively. Adjust the distances a, b, and c between the plates to 30mm, tightly clamp the earthworms; select 15V output, and control the stimulation time to 60s. The rest are ...

technical field

[0001] The invention relates to a device and method for extracting biological body fluids, in particular to a device for rapidly extracting earthworm body fluids by means of electrical stimulation, separating and collecting the body fluids in time, and a matching operation method. Background technique

[0002] Earthworm body fluid is an important part of earthworm respiratory system and immune defense system. Earthworm body fluid is formed by mixing surface fluid and body cavity fluid. Earthworm body surface fluid is a colorless slightly viscous liquid secreted by mucus cells, which can prevent earthworm skin from drying out, dissolve oxygen for earthworm respiration, and excrete N in the body in the form of mucus protein. As an immune barrier, earthworm coelom fluid contains various types of leukocytes and hemolytic proteins, peptides and other biologically active molecules, which have the biological characteristics of selectively inhibiting bacteria and ly...

Examples

Embodiment 1

[0040] The device and method for rapid extraction of electric stimulation type earthworm body fluid in this example refer to Figure 1-Figure 5, Figure 6a1-2. The characteristics of the device in this example are: the upper part of the extraction device is an extraction box, the side of which is equipped with terminal posts, connected to the embedded metal guide rail, the bottom surface is a separation plate, the hole structure adopts the form of a conical counterbore, the hole diameter d is 3mm, and the depth h is 3mm, chamfer angle θ is 20°, hole spacing x and y are both 10mm; 4 L-shaped electrode plates are placed on the metal guide rail, and the metal conductive end and the insulating handle of the adjacent electrode plate are adjacent on the same metal guide rail Placed to form 3 sets of L-shaped plates with adjustable distances. After power-on, the 4 L-shaped plates will show alternating positive and negative voltages; the lower part is the collection box, and the side i...

Embodiment 2

[0042] The device and method for rapid extraction of earthworm body fluid in this example refer to Figure 4 , Figure 5 , Figure 6b1-2. The device described in this example is different from Example 1 in that: the diameter d of the conical counterbore of the separation plate of the extraction device is 4mm, the depth h is 4mm, the chamfer angle θ is 30°, and the hole spacing x and y are 15mm; 4 The T-shaped electrode plates are placed on the metal guide rails to form 3 sets of T-shaped electrode plates with adjustable distances. The method described in this example is different from Example 1 in that: Take 300 g of sexually mature trapezoidal worms (Aporrectodea trapezoides Dugès, 1828) and wash them with clean water, then place them between three groups of plates respectively, and adjust the distance between the plates a, Both b and c are 10mm, and the earthworms are tightly clamped; the 9V output is selected, and the stimulation time is controlled to 45s. The rest are th...

Embodiment 3

[0044] The device and method for rapid extraction of earthworm body fluid in this example refer to Figure 4 , Figure 5 , Figure 6c1-2. The difference between the device described in this example and Embodiment 1 and Embodiment 2 is that the diameter d of the conical counterbore of the separation plate of the extraction device is 5mm, the depth h is 5mm, the chamfer angle θ is 45°, and the hole spacing x and y are both 20mm; 4 E-type electrode plates are placed on the metal guide rail to form 3 sets of E-type electrode plates with adjustable distance. The method described in this example is different from Example 1 and Example 2 in that: take 500 g of sexually mature Amynthas pingi Stephenson (Amynthas pingi Stephenson, 1925) and wash them with clean water and place them between 3 groups of plates respectively. Adjust the distances a, b, and c between the plates to 30mm, tightly clamp the earthworms; select 15V output, and control the stimulation time to 60s. The rest are ...

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