Device and method for quick extraction of electrical stimulation earthworm body fluid
An extraction device and extraction method technology, which are applied in the directions of inoculation and ovulation diagnosis, medical science, and apparatus for restraining animals, etc., can solve the problems of limited extraction target, low extraction efficiency, and unclean separation of glass containers.
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Embodiment 1
[0040] The device and method for rapid extraction of electric stimulation type earthworm body fluid in this example refer to Figure 1-Figure 5, Figure 6a1-2. The characteristics of the device in this example are: the upper part of the extraction device is an extraction box, the side of which is equipped with terminal posts, connected to the embedded metal guide rail, the bottom surface is a separation plate, the hole structure adopts the form of a conical counterbore, the hole diameter d is 3mm, and the depth h is 3mm, chamfer angle θ is 20°, hole spacing x and y are both 10mm; 4 L-shaped electrode plates are placed on the metal guide rail, and the metal conductive end and the insulating handle of the adjacent electrode plate are adjacent on the same metal guide rail Placed to form 3 sets of L-shaped plates with adjustable distances. After power-on, the 4 L-shaped plates will show alternating positive and negative voltages; the lower part is the collection box, and the side i...
Embodiment 2
[0042] The device and method for rapid extraction of earthworm body fluid in this example refer to Figure 4 , Figure 5 , Figure 6b1-2. The device described in this example is different from Example 1 in that: the diameter d of the conical counterbore of the separation plate of the extraction device is 4mm, the depth h is 4mm, the chamfer angle θ is 30°, and the hole spacing x and y are 15mm; 4 The T-shaped electrode plates are placed on the metal guide rails to form 3 sets of T-shaped electrode plates with adjustable distances. The method described in this example is different from Example 1 in that: Take 300 g of sexually mature trapezoidal worms (Aporrectodea trapezoides Dugès, 1828) and wash them with clean water, then place them between three groups of plates respectively, and adjust the distance between the plates a, Both b and c are 10mm, and the earthworms are tightly clamped; the 9V output is selected, and the stimulation time is controlled to 45s. The rest are th...
Embodiment 3
[0044] The device and method for rapid extraction of earthworm body fluid in this example refer to Figure 4 , Figure 5 , Figure 6c1-2. The difference between the device described in this example and Embodiment 1 and Embodiment 2 is that the diameter d of the conical counterbore of the separation plate of the extraction device is 5mm, the depth h is 5mm, the chamfer angle θ is 45°, and the hole spacing x and y are both 20mm; 4 E-type electrode plates are placed on the metal guide rail to form 3 sets of E-type electrode plates with adjustable distance. The method described in this example is different from Example 1 and Example 2 in that: take 500 g of sexually mature Amynthas pingi Stephenson (Amynthas pingi Stephenson, 1925) and wash them with clean water and place them between 3 groups of plates respectively. Adjust the distances a, b, and c between the plates to 30mm, tightly clamp the earthworms; select 15V output, and control the stimulation time to 60s. The rest are ...
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