Improved culture medium for neonatal pig islet cells and use method of same

A technology of islet cells and culture medium, which is applied in the field of improved newborn pig islet cell culture medium, which can solve the problems of unfavorable treatment effect, decreased insulin secretion, difficult to remove pollution, etc., to increase the recovery rate of cultured cells and increase the secretion of insulin , the effect of reducing the number of apoptotic cells

Active Publication Date: 2014-04-02
HUNAN XENO LIFE SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Benefits of technology

This patented technology improves the efficiency at producing beta plasma pealets by increasing their ability to produce various substances called vitamins A or B through oxidizing reactions on carboxyl groups found within certain sugars such as selenium dioxide. These changes help reduce damage caused during storage and transporting processes while maintaining good quality nutritional value.

Problems solved by technology

This technical problem addressed in this patents relates to improving the quality and yielding capacity of producing functional beta type glucose regulatory peptide(BGL). Islet cell therapy offers promise because they offer several advantages over traditional ways like bone marrow grafting. They require fewer sources than older organoids, making them ideal candidates for future use. Additionally, their ability to grow tissue stem cells without damaging themselves could lead to improved treatment outcomes.

Method used

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  • Improved culture medium for neonatal pig islet cells and use method of same
  • Improved culture medium for neonatal pig islet cells and use method of same

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Effect test

Embodiment 1

[0032] Embodiment 1, culture medium of the present invention and culture method test

[0033] The present invention injects 10IU / kg heparin sodium intraperitoneally before taking pancreas from newborn pigs. The inoculation concentration of the present invention is 5000-10000IEQ / 25-30ml for isolated and purified newborn pig islet cells in the culture medium, culture medium (HAM'S F10 Base and added with apoptosis inhibitor Z-VAD-FMK20μΜ, human basic fibroblast growth factor hFGF-β10-20μg / L, vascular endothelial growth factor VEGF10-20μg / L, insulin-like growth factor R3-IGF-110 -20μg / L, human epidermal growth factor hEGF10-20μg / L, hepatocyte growth factor hHGF10-20μg / L and pig serum accounting for 10% of the medium volume, as well as sodium selenite 6.7ng / L, insulin 10μg / L L, transferrin 5.5μg / L, ethanolamine 2μg / L, 1-methyl 3-isobutylxanthine 0.011g / L, nicotine 1.22g / L, cortisol (hydrocortisone) 5μM, 80U / ml penicillin, 100U / ml streptomycin) at 37°C, 5% CO 2 , Cultured in a 95...

Embodiment 2

[0040] Example 2, the effect comparison between the present invention and patent application 201210330427.9, and general cultivation methods, the results are shown in Table 1-3.

[0041] The survival rate of active islet cells cultured in the present invention for 6 days reaches 97.68±1.75%, which is higher than that of general cultured islet cells (<70%) and the original patent 201210330427.9 (≤82%); the survival rate of active pancreatic islet cells cultivated in the present invention for 10 days reaches 88.9% ±1.63%, much higher than the general medium (<10%) and the original patent 201210330427.9 (69.20%); the percentage of β cells cultured for 6 days in the present invention is 85.25±1.69%, which is also higher than the general medium (<50%) and the original patent 201210330427.9 (≤72.0%); the percentage of β cells cultured for 10 days in the present invention reaches 96.8±0.45%, which is higher than that of ordinary culture medium (<60%) and the original patent 2012103304...

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Abstract

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

Description

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Claims

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Application Information

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Owner HUNAN XENO LIFE SCI
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