Method for rapidly identifying functions of pepper fruit color development related genes

Abstract

The invention discloses a method for rapidly identifying functions of pepper fruit color development related genes. The virus induced gene silencing technology and the detached pepper fruit color change are combined to carry out functional identification to pepper fruit color development related genes and pepper fruit color development unrelated genes. The method mainly comprises the steps of constructing a gene silencing expression vector, using agrobacterium GV3101 to mediate and infect detached pepper fruits and observing the change of fruit color phenotype, wherein the detached pepper fruits with the silencing effect have obvious orange or yellow symptoms, and the situation shows that the detected genes are related to the pepper fruit color development; on the contrary, the color of the fruit peel is changed normally or the fruit peel color has no obvious change, and the situation shows that the detected genes are unrelated to the pepper fruit color development or are non-functional genes. The method provides convenience for rapidly identifying the functions of a great amount of pepper fruit color development related genes and can greatly accelerate the pepper fruit molecular breeding process.

Description

technical field [0001] The invention belongs to the field of plant molecular breeding, and in particular relates to a method for identifying the function of genes related to fruit color development of capsicum fruit by using virus-induced gene silencing technology. Background technique [0002] Pepper (Capsicum annuum L.) belongs to Solanaceae (Solanaceae) Capsicum (Capsicum), an annual or perennial herbaceous plant, native to the tropical and subtropical regions of Central and South America, and now it is widely cultivated all over the world, and it is also one of the vegetable crops with the largest planting area in my country. one. [0003] With the advent of the genome era and the transcriptome era, a large number of gene functions need to be quickly identified, while the traditional transgenic technology is complex in operation, long in cycle, and low in conversion rate, which seriously restricts the identification of gene functions. For this reason, the present inventi...

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Problems solved by technology

[0006] Fu et al. (2005) successfully injected tobacco rattle virus (TRV) carrying the target gene fragment into tomato fruits growing on plants through VIGS technology, and tested the function of the ethylene gene controlling tomato fruits. Vacuum infiltration method was used to conduct VIGS experiments on fruits, but the efficiency of vacuum infiltration method was low, and most of the fruits rotted after infiltration. The

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