Chemiluminescence detection kit for furaltadone metabolite and applications of the kit

A technology of chemiluminescence detection and furatanone, applied in the field of immunological detection, can solve the problems of cumbersome process, slow detection speed, easy occurrence of false positive results, etc., and achieve the effect of high sensitivity
CN103698519AInactive Publication Date: 2014-04-02BEIJING KWINBON BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING KWINBON BIOTECH
Publication Date
2014-04-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a chemiluminescence detection kit (CLEIA) for a furaltadone metabolite (AMOZ). The kit comprises a kit body, a chemiluminescence plate disposed in the kit body and reagents disposed in the kit body. Apertures of the chemiluminescence plate are coated with an AMOZ coupling antigen. The reagents comprise enzyme labeled antibody concentrate, enzyme labeled antibody diluent, AMOZ series standard substance solution, chemiluminescence A liquid, chemiluminescence B liquid, concentrated washing liquid, concentrated redissolution liquid, and a derivatization agent. The detection kit has characteristics of high sensitivity, simpleness, rapidness and high accuracy. Compared with a traditional ELISA method, the operation time of the detection kit provide by the invention is largely reduced. The detection kit can be used for tissue (muscle and liver) detection of livestock and poultry, and used for AMOZ residue detection in aquatic products.
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Description

technical field

[0001] The invention relates to a chemiluminescent immunoassay kit for detecting furaltadone metabolites (AMOZ), which is used for detecting the AMOZ content or residue in livestock and poultry tissues (muscle, liver) and aquatic products. It belongs to the field of immunological detection. Background technique

[0002] Furaltadone belongs to nitrofuran antibacterial drugs, and it is mainly used clinically for the treatment of various intestinal infectious diseases such as pullorum, chicken colibacillosis, chicken coccidiosis and as a growth-promoting agent for pigs, poultry and aquatic products. additive. However, relevant studies have proved that furaltadone and its metabolites have considerable toxicity and side effects, can induce genetic mutations and teratogenicity in organisms, and can induce cancer.

[0003] The European Union promulgated Regulation 2377 / 90 / EEC as early as 1990, listing nitrofuran drugs and their metabolites as Class A banned drugs,...

Claims

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