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Kit for quantitatively detecting alpha-glucan by monoclonal antibody immunonephelometry

A technology for quantitative detection of glucan, applied in measuring devices, material analysis by observing the influence on chemical indicators, instruments, etc., can solve the problem of lack of performance antibodies and kits for quantitative detection of α-glucan and other problems, to achieve the effect of simple test conditions, high sensitivity, and easy operation

Inactive Publication Date: 2014-04-02
GUANGZHOU SUGARCANE IND RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of antibodies with excellent performance in the prior art, and there is no practical kit for the quantitative detection of α-glucan

Method used

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  • Kit for quantitatively detecting alpha-glucan by monoclonal antibody immunonephelometry
  • Kit for quantitatively detecting alpha-glucan by monoclonal antibody immunonephelometry
  • Kit for quantitatively detecting alpha-glucan by monoclonal antibody immunonephelometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The composition of each reagent is as follows:

[0041] 1. Sample diluent

[0042] Phosphate (buffer) 100mmol / L (pH7.2)

[0043] PEG6000 (surfactant) 5%

[0044] KCl (electrolyte) 10%

[0045] 2. Antibody diluent

[0046] Phosphate (buffer) 100mmol / L (pH7.2)

[0047] KCl (electrolyte) 5%

[0048] 3. Standard product

[0049] Phosphate (buffer) 100mmol / L (pH7.2)

[0050] PEG6000 (surfactant) 5%

[0051] KCl (electrolyte) 10%

[0052] Dextran T-2000500mg / L

[0053] 4. Antibody freeze-dried powder

[0054] Dextran Antibody 20mg

[0055] In this example, a high-concentration single-point reference standard was selected, and the concentration of dextran T-2000 was added at 500 mg / L, then filtered with a 0.45 μm filter membrane, and stored at 2-8°C. When used, dilute the standard with different concentration gradients with the sample diluent, and draw the standard curve. Take 10mL of the antibody diluent and mix it with the antibody freeze-dried powder, shake wel...

Embodiment 2

[0057] The composition of each reagent is as follows:

[0058] 1. Sample diluent

[0059] Phosphate (buffer) 50mmol / L (pH7.4)

[0060] Dodecyltrimethylammonium chloride 0.5%

[0061] NaCl (electrolyte) 10%

[0062]2. Antibody diluent

[0063] Phosphate (buffer) 50mmol / L (pH7.4)

[0064] NaCl (electrolyte) 5%

[0065] 3. Standard product

[0066] Phosphate (buffer) 50mmol / L (pH7.4)

[0067] Dodecyltrimethylammonium chloride 0.5%

[0068] NaCl (electrolyte) 10%

[0069] Dextran T-2000500mg / L

[0070] 4. Antibody freeze-dried powder

[0071] Dextran Antibody 20mg

[0072] In this example, a high-concentration single-point reference standard was selected, and the concentration of dextran T-2000 was added at 500 mg / L, then filtered with a 0.45 μm filter membrane, and stored at 2-8°C. When used, dilute the standard with different concentration gradients with the sample diluent, and draw the standard curve. Take 10mL of the antibody diluent and mix it with the antibody f...

Embodiment 3

[0074] The composition of each reagent is as follows:

[0075] 1. Sample diluent

[0076] Phosphate (buffer) 100mmol / L (pH6.8)

[0077] Tween-80 (surfactant) 5%

[0078] KCl (electrolyte) 10%

[0079] 2. Antibody diluent

[0080] Phosphate (buffer) 100mmol / L (pH6.8)

[0081] KCl (electrolyte) 5%

[0082] 3. Standard product

[0083] Phosphate (buffer) 100mmol / L (pH6.8)

[0084] Tween-80 (surfactant) 5%

[0085] KCl (electrolyte) 10%

[0086] Dextran T-2000500mg / L

[0087] 4. Antibody freeze-dried powder

[0088] Dextran Antibody 20mg

[0089] In this example, a high-concentration single-point reference standard was selected, and the concentration of dextran T-2000 was added at 500 mg / L, then filtered with a 0.45 μm filter membrane, and stored at 2-8°C. When used, dilute the standard with different concentration gradients with the sample diluent, and draw the standard curve. Take 10mL of the antibody diluent and mix it with the antibody freeze-dried powder, shake w...

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Abstract

The invention discloses a kit for quantitatively detecting alpha-glucan by monoclonal antibody immunonephelometry. The kit comprises alpha-glucan antibody freeze-drying powder, an antibody diluent, a sample diluent and a standard substance, wherein the alpha-glucan antibody freeze-drying powder is freeze-drying powder of IgM type monoclonal antibody purified in mouse ascites where a hybridoma cell strain D9 with a preservation number of CCTCCC2010107 (China Center for Type Culture Collection C2010107) is injected. The quantitative detection kit provided by the invention has the characteristics of simpliness and convenience in operation, good repeatability, high sensitivity and simple test conditions, is suitable for detection of an alpha-glucan immune complex separated out from a determination reaction system, and is suitable for sugaring processes of cane juice, raw sugar, white granulated sugar, brown granulated sugar, syrup, molasses and the like.

Description

technical field [0001] The invention relates to a polymer quantitative detection kit, in particular to a monoclonal antibody immune turbidimetric quantitative detection kit for α-glucan. Background technique [0002] α-glucan is a polysaccharide composed entirely of glucose monomers, the molecular formula ((C 6 h 10 o 5 ) n ), the molecular weight is generally between tens of thousands to several million, and it is usually a white amorphous powder solid, which is sticky, and the viscosity increases with the increase of molecular weight and concentration. decrease with increasing molecular weight. It can exist stably in normal temperature and neutral solution, and it will decompose when encountering strong acid, and its end group will be easily oxidized in alkaline solution, and it can gradually change color or decompose when heated. [0003] From sugar cane juice to refined products, there are adverse effects caused by dextran from the beginning to the end in the sugar ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/82
CPCG01N33/577G01N21/82
Inventor 梁达奉曾练强柳颖蚁细苗马步林荣珍黄曾慰李雨虹
Owner GUANGZHOU SUGARCANE IND RES INST
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