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Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker

A Hong Kong oyster and freeness technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of no high-yield, high-quality and stress-resistant new varieties, etc., and achieve the effect of significant breeding advantages

Active Publication Date: 2014-04-30
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
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Problems solved by technology

At present, the development of this industry is almost entirely cultivated by natural seedlings, and there are no new high-yielding, high-quality and stress-resistant varieties

Method used

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  • Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker
  • Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker
  • Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker

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Embodiment 1

[0015] (a) Classification: On April 28, 2010, 1,000 wild 2-year-old Hong Kong oysters from Shenzhen were used as the basic population. The right shell of the oyster was opened, and then the top of the shell was gently peeled off with tools such as oyster knives and tweezers. From the mantle between the adductor muscles, four rows of free gill tubes were found in Hong Kong oysters, and the number of free gill openings of each oyster is different, such as figure 2 Individual A has 6 free gill openings, while figure 2 B is a group of oysters that are not free at all (such as near river oysters, long oysters, Portuguese oysters, Kumamoto oysters, etc.). According to the technical route figure 1 , with the number of free gill openings as markers, divide the freeness of gill openings into highly free groups (number of gill openings ≥ 9, figure 2 C), moderately free taxa (5 figure 2 D), low free taxa (number of gill openings ≤ 5, figure 2 E) 3 taxa. In taxa division, the prop...

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Abstract

The invention discloses a method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as a marker. According to the method, wild 2-year-old oysters of Crassostrea hongkongensis are taken as a basic population; dividing is performed using a number of free gill apertures as a marker; if the number of apertures is not smaller than 9, a highly free group is determined; Crassostrea hongkongensis individuals in the highly free population are used as a breeding population; group self-breeding is performed by means of gamete dissection; seed breeding and cultivation is then performed to obtain a first filial generation of the highly free group; the number of free gill apertures is continually used as a directional selection standard; the first filial generation of the highly free group is used as a parent breeding second filial generation; after the cycle goes on for generations, a new strain of Crassostrea hongkongensis having stably-inherited bill aperture freeness and excellent phenotypic character is obtained. The unique gill aperture freeness in Crassostrea hongkongensis is used as the marker, three grouping standards are determined for the first time, quantitative character and economic character are collaboratively selected, and the new strain of Crassostrea hongkongensis having significant culture advantages is obtained by directional purification.

Description

Technical field: [0001] The invention belongs to the field of shellfish genetics and breeding in marine agriculture, and in particular relates to a seed production method of Hong Kong oysters marked with gill hole freeness. Background technique: [0002] Hong Kong oyster Crassostrea hongkongensis is the main economic species of shellfish cultured in the coastal areas of South my country. It likes to live in brackish water areas near estuaries or nearby freshwater injection. It is mainly distributed in Guangdong, Guangxi and other places, with an annual output of more than 1.3 million tons. The output value is 6-8 billion yuan (China Fishery Yearbook, 2013). At present, the development of this industry is almost entirely cultivated by natural seedlings, and there are no new high-yielding, high-quality and stress-resistant varieties. [0003] The main economic oyster along the coast of South my country, formerly known as Omi oyster C. rivularis, has two "types", one is "white ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张跃环喻子牛肖述张扬
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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