Acremonium terricola mutant strain and application thereof

A technique of acremonium acremonium and mutagenic strains, applied in the field of microbial germplasm resource utilization, can solve the problems of low content of secondary metabolite adenosine and limit the utilization of acremonium acremonium, so as to improve production performance and health The effect of the situation

Active Publication Date: 2014-04-30
合肥迈可罗生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the content of secondary metabolites adenosine, sterols, crude protein and polysaccharides in the current

Method used

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  • Acremonium terricola mutant strain and application thereof
  • Acremonium terricola mutant strain and application thereof
  • Acremonium terricola mutant strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1 Mutagenesis Screening Obtained Mutant Strain of Acremonium

[0036] 1 Starting strain isolation

[0037] (1) Cordyceps collection

[0038] Cordyceps was collected from Sangongjian, Wenquan Town, Yuexi County, Anhui Province, with fresh sub-seats. It has been identified as a species of Cordyceps gunnii var.minor.

[0039] (2) Isolation, purification and identification of Cordyceps fungus

[0040] Under aseptic conditions, rinse the fresh cordyceps stalk thoroughly with sterile water, then soak it with 0.5% chloramine T for 15 minutes, and then rinse thoroughly with sterile water; use a sterile scalpel to carefully cut the cordyceps stalk longitudinally Cut into 4 petals and place them in 4 plates covered with SDAY medium; culture at 20°C for about two weeks until visible colonies grow; use an inoculating needle to pick out the colony hyphae and transfer them to another fresh SADY plate, 20 Cultured at ℃ for about a week to grow a single purified colony; pick the purif...

Example Embodiment

[0098] Example 2 Preparation of feed additives using Acremonium terricola MKL18 mutant

[0099] 1 prepare seed liquid

[0100] Take the Acremonium terricola mutant strain (Acremonium terricola MKL18) preserved at 4°C, inoculate it on the PDA slant, and culture for 7 days at 26°C; transfer the slant colony to a 1000ml Erlenmeyer flask containing 200ml of liquid Cha's medium, 26 Cultivate with shaking at 125r / min for 4 days; transfer the culture solution once again according to the 10% inoculum amount, and culture with shaking for 4 to 5 days under the same conditions to obtain seed solution.

[0101] 2 liquid fermentation

[0102] In a 50L air-lift fermenter, add 30L of fermentation broth, and add an appropriate amount of soaked enemy, keep the temperature at 115°C for 20 minutes; when the temperature drops to 26°C, insert 600ml of seed liquid; keep the tank temperature at 26°C, and ventilate The amount is 15L / min, the fermentation is 48h and put into the tank to obtain the first-leve...

Example Embodiment

[0119] Example 3 Effect of feed additives on growth performance and health status of piglets

[0120] Select 200 healthy Du-Long-Large hybrid piglets 30 days old and similar in weight (7.95±0.87kg), half male and half female, and randomly divided into 4 groups according to the principle of similar size and sex, each with 50 pigs;

[0121] The feed additives (solid substrate of formula 2) prepared in Example 2 by fermentation of the mutant strain were added to the basic diet at the ratios of 0.25%, 0.5%, and 0.75%, respectively, and three groups of piglets were fed respectively for experiment Group: One group of piglets was fed directly with the basal diet, as the control group. Observe and record the growth performance and health status of the piglets in the test group and the control group. The results are shown in Table 5 and Table 6, respectively.

[0122] Table 5 Effects of feed additives on growth performance of piglets

[0123]

[0124] Note: a and b respectively indicate the s...

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Abstract

The invention discloses an acremonium terricola mutant strain and an application thereof. The collection number of the acremonium terricola mutant strain (MKL18) is CCTCC No.M2013654; and the application refers to the application thereof in preparing feed additives, drugs and healthcare products. In the invention, the acremonium terricola MKL18 is obtained through ultraviolet mutation screening, the mycelium extract thereof has in-vitro anti-tumor activity and a function of enhancing the in-vivo immunocompetence of an animal and can be used for preparing drugs and healthcare food; the acremonium terricola MKL18 is subjected to liquid-solid two-phase fermentation, and the fermentation product is dried and ground and then can be directly used as a feed additive; and the feed additive remarkably improves the productivity of weaned piglets and remarkably improves the health state of the weaned piglets.

Description

technical field [0001] The invention belongs to the technical field of utilization of microbial germplasm resources, and in particular relates to a mutagenic strain of Acremonium acremonium and its application. Background technique [0002] Cordyceps is a biological complex formed by fungi infecting insect larvae. Currently, there are more than 350 species of Cordyceps found in the world, and more than 70 species have been found in China alone. Among them, the most famous Cordyceps sinensis has long been regarded as a precious traditional Chinese medicine, and scientific research has also confirmed that Cordyceps sinensis contains a variety of pharmacologically active ingredients. [0003] Among the secondary metabolites of Cordyceps and related fungi, components such as adenosine, sterols, and polysaccharides are related to many pharmacological activities of Cordyceps. Among them, adenosine has a variety of biological activities, including sedative, blood vessel expansion ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23K1/16A61K36/068A61P35/00A61P37/04A23L1/29C12R1/645A23L33/00
Inventor 李晓祥桂向东李德刚徐自奥
Owner 合肥迈可罗生物工程有限公司
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