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Actinomycetes with broad-spectrum antimicrobial activity and screening method thereof

A technology of antibacterial activity and screening method, which is applied in the field of microorganisms, can solve the problems of unsatisfactory control effect, unfavorable production of pollution-free green fruit, polluted soil environment, etc., and achieve the effects of pollution-free, significant control effect, and no soil pollution

Inactive Publication Date: 2014-05-14
BEIJING UNIV OF AGRI
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Problems solved by technology

[0004] However, at present, these methods of controlling plant diseases with chemical pesticides have unsatisfactory control effects, and the use of chemical pesticides to prevent and control plant diseases will pollute the soil environment, which is not conducive to pollution-free and green fruit production

Method used

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  • Actinomycetes with broad-spectrum antimicrobial activity and screening method thereof
  • Actinomycetes with broad-spectrum antimicrobial activity and screening method thereof
  • Actinomycetes with broad-spectrum antimicrobial activity and screening method thereof

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Embodiment Construction

[0013] The present invention will be further described in detail below through specific implementation examples in conjunction with the accompanying drawings.

[0014] The first embodiment of the present invention provides an actinomycete with broad-spectrum antibacterial activity. The bacterium is Streptomyces sp., which has been deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee on March 5, 2013, and is the depositary unit The address is: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; the deposit number is CGMCC No. 7278; its classification is named: Streptomyces sp. Experimental data shows that Streptomyces sp. is effective against plum dry rot pathogen Polvporus hirsutus, Botrytiscinerea Botrytis cinerea Phomopsis vexans, Rhizopus nigricans, Potato dry rot pathogen Fusarium caerulem (Libert) Sacc. , Alternaria brassicae, Verticillium dahlia, Alternari...

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Abstract

The invention relates to the technical field of microorganisms, and specifically, particularly relates to actinomycetes with broad-spectrum antimicrobial activity and a screening method thereof. The actinomycetes with broad-spectrum antimicrobial activity belong to Streptomyces sp, and the preservation number is CGMCC No.7278. The screening method of the actinomycetes with broad-spectrum antimicrobial activity comprises the following steps: obtaining cultivated streptomyces from a to-be-separated soil sample; screening to obtain actinomycetes of antagonism target bacteria from the cultivated actinomycetes by using a plate confrontation method, an inhibition zone method or a cross patch method. By adopting the actinomycetes with broad-spectrum antimicrobial activity and the screening method thereof provided by the invention, the prevention role on the plant disease is played by inhibiting the growth and propagation action of the pathogenic bacteria. Compared with the traditional chemical pesticide actinomycetes for preventing and treating the plant disease, the method is significant in prevention and treatment effects, free of pollution to soil, and beneficial to production of pollution-free green products.

Description

Technical field [0001] The present invention relates to the field of microbial technology, in particular, to actinomycetes with broad-spectrum antibacterial activity and a screening method thereof. Background technique [0002] Plant diseases are caused by a variety of pathogens. For example, peach root cancer (also known as crown gall disease) is a worldwide plant bacterial tumor disease caused by Agrobacterium tumefaciens. It occurs in tree cultivation areas, and in some areas, the incidence rate is as high as 100%; once a plant is infected, it is difficult to cure, ranging from weak yellow leaves to dead trees; plum dry rot is an important disease that harms branches and fruits , Is an important reason for the low survival rate of plum planting and the death of young trees. The pathogen is Polvporus hirsutus. In addition to plum trees, it also harms pears, citrus, stone fruit trees, some dried fruit species, and poplar, Willow, locust and other forest trees; Strawberry Botryt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12Q1/04C12R1/465
Inventor 刘志民马焕普薛红燕
Owner BEIJING UNIV OF AGRI
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