Multiplex amplification kit of 18 short tandem repeats (STR)

A technology of compound amplification and compound amplification system, which is applied to the detection of genetic marker genes with polymorphism in the human genome, and the simultaneous amplification of multiple short tandem repeat loci. Standard, incompatibility, insufficient processing and other problems, to achieve the effect of saving cost and manpower, improving work efficiency, and high individual recognition rate
CN103789413AInactive Publication Date: 2014-05-14BEIJING PEOPLESPOT TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING PEOPLESPOT TECH
Publication Date
2014-05-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a multiplex amplification kit of 18 short tandem repeats (STR), relating to a multiplex amplification system which simultaneously analyzes a plurality of STR loca. The multiplex amplification kit is characterized by carrying out multiplex amplification on the following 18 loca: Amelogenin, D6S477, GATA198B05, D15S659, D8S1332, D3S3045, D17S1290, D14S608, D2S441, D18S535, D13S325, D10S1435, D11S2368, D1S1656, D7S3048, D10S1248, D19S253 and D3S1358. The invention also relates to a method and kit for simultaneously analyzing DNA (deoxyribonucleic acid) samples and applications thereof.
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Description

Technical field:

[0001] The present invention relates to the field of biotechnology. Further, the present invention relates to the detection of polymorphic genetic marker genes in the human genome. In particular, the present invention relates to the simultaneous amplification of multiple short tandem repeat loci in one amplification system using polymerase chain reaction. Background technique:

[0002] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large, the division is extensive, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high, and its polymorphism is mainly derived from the difference in the number of repeats of the core sequence between individuals. And this difference follows the Mendelian ...

Claims

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