Multiplex amplification kit of 18 short tandem repeats (STR)

A technology of compound amplification and compound amplification system, which is applied to the detection of genetic marker genes with polymorphism in the human genome, and the simultaneous amplification of multiple short tandem repeat loci. Standard, incompatibility, insufficient processing and other problems, to achieve the effect of saving cost and manpower, improving work efficiency, and high individual recognition rate

Inactive Publication Date: 2014-05-14
BEIJING PEOPLESPOT TECH
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Problems solved by technology

In order to avoid incompatibility between kits, the loci selected to be added are basically the same, which leads to the fact that even if kits from different manufacturers are used, only information on up to 23 loci can be obtained
And 23 loci information is not enough to deal with some of the problems that often arise in paternity testing
[0005] There is no unified standard for the judgment of paternity test in the world

Method used

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  • Multiplex amplification kit of 18 short tandem repeats (STR)
  • Multiplex amplification kit of 18 short tandem repeats (STR)
  • Multiplex amplification kit of 18 short tandem repeats (STR)

Examples

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Embodiment 1

[0046] Example 1 multiplex amplification of 18 gene loci and analysis of their genotypes

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Abstract

The invention provides a multiplex amplification kit of 18 short tandem repeats (STR), relating to a multiplex amplification system which simultaneously analyzes a plurality of STR loca. The multiplex amplification kit is characterized by carrying out multiplex amplification on the following 18 loca: Amelogenin, D6S477, GATA198B05, D15S659, D8S1332, D3S3045, D17S1290, D14S608, D2S441, D18S535, D13S325, D10S1435, D11S2368, D1S1656, D7S3048, D10S1248, D19S253 and D3S1358. The invention also relates to a method and kit for simultaneously analyzing DNA (deoxyribonucleic acid) samples and applications thereof.

Description

Technical field: [0001] The present invention relates to the field of biotechnology. Further, the present invention relates to the detection of polymorphic genetic marker genes in the human genome. In particular, the present invention relates to the simultaneous amplification of multiple short tandem repeat loci in one amplification system using polymerase chain reaction. Background technique: [0002] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large, the division is extensive, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high, and its polymorphism is mainly derived from the difference in the number of repeats of the core sequence between individuals. And this difference follows the Mendelian ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 张威张琰
Owner BEIJING PEOPLESPOT TECH
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