Rapid extraction method of dry tobacco leaf DNA

Abstract

The invention discloses a rapid extraction method of dry tobacco leaf DNA, comprising steps of material drawing, pretreatment and extraction. The rapid extraction method specifically comprises the following steps: numbering ventilating ziplock bags, picking tender leaves from the bottom of tobacco plants, and putting the tender leaves into the ziplock bags;?putting?the?ziplock bags?into?vacuum pumping pockets, adding?silica gel?into the?pockets and placing the?pockets in a cool place and / or a drying machine / cabinet?until?moisture content?is 1-3%, sealing,?extracting the air?to obtain?dry tobacco leaves;?grinding the obtained?dry tobacco leaves, adding a DNA extraction buffer?and DDT, uniformly mixing, carrying out?thermostatic waterbath, adding?a sodium acetate solution,?uniformly blending?by?oscillation, taking a?supernate?a after centrifugation, taking a supernate?b after second?centrifugation, adding?isopropyl alcohol?of equal volume,?centrifuging and discarding a?supernate?c, dissolving?and washing a?precipitate by adding 70%?alcohol?into the?precipitate,?centrifuging and discarding a supernate?d, adding?absolute ethyl alcohol?into the?precipitate?to wash the?precipitate, drying, dissolving?the precipitate by adding redistilled water into the precipitate, and carrying out electrophoresis detection. According to the invention, the method is simple; the operation is simple and rapid; and DNA extraction efficiency?of a sample is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for rapidly extracting DNA from dried tobacco leaves. Background technique [0002] Molecular biology techniques have penetrated into various aspects of biology, medicine, botany, genetics and zoology. The quality and yield of DNA extraction are key steps that directly affect PCR amplification. Since the advent of PCR technology in 1985, this technology has been applied to various information fields of life sciences because of its high sensitivity, strong specificity, and easy operation. , short time required and other advantages have been widely used in a variety of detection fields. [0003] In the process of DNA extraction, the extracted sample has a very important impact on the final result, and the samples cannot be stored for a long time and cannot be traced back. At present, there is no relatively simple, fast and long-term storage method f...

AI Technical Summary

Problems solved by technology

[0003] In the process of DNA extraction, the extracted sample has a very important impact on the final result, and the samples cannot be stored for a long time, and cannot be traced

At present, there is no relatively simple, fast and long-term storage method for the preservation of samples, which cannot fundamentally solve the problem of preservation and traceability of extracted samples.

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