Method for detecting effective ingredients of isodon lophanthoides

The technology of the fenugreek and the detection method is applied in the field of detection of the active ingredients of the fenugreek fenugreek, which can solve the problems of uncollected and undiscovered, and achieves small error in results, good specificity and quality correlation , Easy to operate effect

Active Publication Date: 2014-06-11
GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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Problems solved by technology

At present, although Xianhuaxianwen fragrant tea dishes are widely used, but after consulting a large amount of information, it is found that none of the previous editions of the "Chinese Pharmacopoeia"
The quality identification of the fibrous and thread-like fragrant tea vegetables recorded in the 2011 edition of "Guangdong Provincial Standards of Traditional Chinese Medicinal Materials" and "Guangxi Provincial Standards of Traditional Chinese Medicinal Materials" is mainly through the inspection of their properties and identification by TLC. It is difficult to rely solely on properties and TLC identification. Determining the content of active ingredients in Xianhuaxianwen fragrant tea, and identifying its quality, but no literature reports on the active ingredients in Xianhuaxianwen fragrant tea have been found so far

Method used

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  • Method for detecting effective ingredients of isodon lophanthoides
  • Method for detecting effective ingredients of isodon lophanthoides
  • Method for detecting effective ingredients of isodon lophanthoides

Examples

Experimental program
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Effect test

Embodiment 1

[0066] Determination of the content of iso-schaftoside, schaftoside and rosmarinic acid in Xianhuaxianwen fragrant tea by high performance liquid chromatography:

[0067] 1) Prepare the reference solution:

[0068] Accurately weigh 10 mg of isoschaftoside reference substance, put it in a 100ml volumetric flask, add a small amount of 70% (volume) methanol aqueous solution to dissolve, set the volume to the mark, shake well, and prepare isoxafotoside with a concentration of 0.15 mg / ml Tacoside reference substance solution, and stored in a refrigerator at 4°C for later use.

[0069] The same method was used to prepare the reference substance solution of schiaftoside and rosmarinic acid.

[0070] 2) Prepare the test solution:

[0071] Take 1g of Xianhuaxianwen Fragrant Tea Vegetables medicinal material, accurately weigh it, put it in a 100ml conical flask, add 25ml of 70% (volume) methanol aqueous solution precisely, weigh it, extract it by ultrasonic for 30min, after cooling, a...

Embodiment 2

[0083] The methodological investigation of the detection method of the active ingredient of the fiber flower thread pattern fragrant tea dish of the present invention:

[0084] (1) Linear relationship

[0085] Using the same operating steps and conditions as in Example 1, accurately measure the appropriate amount of iso-schafutaside, schafutaside and rosmarinic acid reference solution respectively, inject a sample for analysis, and use the injection volume to the integral value of the chromatographic peak area Perform linear regression processing to obtain the regression line equations, correlation coefficients and linear ranges of isoschaftosides, schafutasides and rosmarinic acid, as shown in Table 1. The results show that the three are within the corresponding ranges and have good linearity relationship, see Figure 7 , Figure 8 , Figure 9 .

[0086] Table 1: Linear inspection experimental results

[0087]

[0088] (2) Precision experiment

[0089] Using the same...

Embodiment 3

[0105] Selection and optimization of gradient elution conditions:

[0106] (1) Gradient elution conditions: 0~30min, mobile phase A is 25%, mobile phase B is 75%;

[0107] 30~70 min, the mobile phase A is 25%→40%, and the mobile phase B is 75%→60%. See image 3 .

[0108] (2) Gradient elution conditions: 0-70 min, 40% mobile phase A, 60% mobile phase B. See Figure 4 .

[0109] From image 3 and Figure 4 It can be seen that the above gradient elution conditions (1) and (2) cannot separate the target peaks well

[0110] (3) Gradient elution conditions: 0~30min, mobile phase A is 30%, mobile phase B is 70%;

[0111] 30~70 min, the mobile phase A is 30%→50%, and the mobile phase B is 70%→50%. See Figure 5 .

[0112] From Figure 5 It can be seen that the target peak can be separated better, so this elution condition was chosen.

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Abstract

The invention provides a method for detecting effective ingredients of isodon lophanthoides. According to the detection method, the content of isoschaftoside, isoschaftoside and rosmarinic acid in isodon lophanthoides is determined by employing HPLC; and the conditions of HPLC comprise: a C18 reversed phase chromatographic column employing octadecylsilane bonded silica as a filling agent is employed, the mobile phase comprises a mobile phase A which is methanol, and a mobile phase B which is a 0.1% (volume) aqueous solution of phosphoric acid, and gradient elution is performed; the program of gradient elution comprises: 0-30 min, 30% of the mobile phase A and 70% of the mobile phase B; 30-70 min, 30% -> 50% of the mobile phase A, and 70% -> 50% of the mobile phase B, wherein the ratio of the mobile phases employs the volume percentage. The detection method successively helps to realize simultaneous determination on content of effective ingredients flavonoids and rosmarinic acid in isodon lophanthoides by employing HPLC, and the data can be used to characterize the medicinal material quality of isodon lophanthoides. A chromatogram of the content of isoschaftoside, isoschaftoside and rosmarinic acid is shown in the specification.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicinal materials, and in particular relates to a method for detection of effective components of fragrant tea and vegetables with fibrous flowers and threads. Background technique [0002] Isodon lophanthoides (Buch. -Ham. ex D. Don) Hara var. graciliflora (Benth.) H. Hara is the source of Chinese medicine Xihuangcao One, it has the effects of clearing heat and dampness, reducing jaundice and dampness, cooling blood and dissipating blood stasis, and can be used to treat acute jaundice hepatitis, acute cholecystitis, dysentery, enteritis and other diseases. It is a commonly used medicine for treating hepatitis B in southern my country. herbs. At present, although Xianhuaxianwen fragrant tea dishes are widely used, it is found that none of the previous editions of the "Chinese Pharmacopoeia" has been included in a large amount of information. The quality identification of the fibro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 匡艳辉林青黄琳姚小华王德勤
Owner GUANGZHOU BAIYUSN HUTCHISON WHAMPOA CHINESE MEDICINE
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