Natto Jiejiu Oral Liquid and Its Application
A kind of wine oral liquid and technology of oral liquid, applied in the field of preparation of natto anti-alcoholic oral liquid, can solve problems such as limited effect, achieve the effects of taking convenience, improving liver function, and alleviating drunkenness
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[0020] 1. Experimental materials
[0021] Bacillus natto, soybean 500g, kudzu flower 90g, Hovenia dulcis 90g, chrysanthemum 90g, hawthorn 120g
[0022] 0.85% physiological saline, Jiejiuling oral liquid (Hangzhou Baisui Biotechnology Co., Ltd.), 56-degree Red Star Erguotou (Beijing Red Star Co., Ltd.)
[0023] KM mice 25-27g (half male and half female)
[0024] 2. Instrument
[0025] Syringe gavage needle, syringe, stopwatch
[0026] 3. Experimental method and result analysis
[0027] 3.1 Natto production method
[0028] 3.1.1 Strain activation: put 100ul into a test tube containing 10ml LB liquid medium, culture at 37°C, 200r / min for 10h-12h.
[0029] 3.1.2 Seed solution preparation: 100ml LB liquid medium, inoculated with 100uL Bacillus natto, cultured at 37°C, 180r / min for 20h.
[0030] 3.1.3 Natto production: Soak at 35°C for about 3 hours, until 90% of the beans are swollen and wrinkle-free, take out and drain, cook for 60 minutes, ferment at 37°C for 24 hours after...
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