Colloidal gold immunochromatographic test paper for detecting tobacco mosaic virus and preparation method thereof
A technology of tobacco mosaic virus and immunochromatographic test paper, which is applied in the agricultural field and can solve the problems of expensive antiserum, few varieties, and insufficient sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] Preparation of colloidal gold immunochromatographic test paper for detecting tobacco mosaic virus.
[0032] A. Preparation of Tobacco Mosaic Virus-labeled Antibody:
[0033] 1) Prepare 1% chloroauric acid solution: weigh 1g of chloroauric acid and dissolve it in 90ml of deionized water, set the volume of the volumetric flask to 100ml, then filter it with a 0.22μm filter membrane, and store it in the dark at 4°C;
[0034] 2) Prepare 1% trisodium citrate solution: weigh 1g of trisodium citrate and dissolve it in 90ml deionized water, set the volumetric flask to 100ml, and then filter it with a 0.22μm filter membrane;
[0035] 3) Prepare 0.1M potassium carbonate solution: weigh 1.38g of potassium carbonate and dissolve it in 90ml of deionized water, set the volume to 100ml, and then filter with a 0.22μm filter membrane;
[0036] 4) Prepare colloidal gold by trisodium citrate reduction method: Measure 495ml of deionized water with a graduated cylinder, pour it into a glass...
Embodiment 2
[0045] The sensitivity of the colloidal gold immunochromatographic test paper prepared above for the detection of tobacco mosaic virus was tested.
[0046] Measure the purified sample of tobacco mosaic virus, choose 0.02M PBS (pH=7.4) buffer solution for dilution, and prepare the concentration as 0ng / ml, 0.1ng / ml, 1ng / ml, 10ng / ml, 100ng / ml, 1000ng / ml The tobacco mosaic virus standard solution of ml is detected using the colloidal gold immunochromatographic test paper for detecting tobacco mosaic virus obtained in Example 1; observe the detection result in 10 minutes; its detection result is as shown in table 1.
[0047] Table 1 Test results of different concentrations of tobacco mosaic virus samples
[0048] Concentration (ng / m) 0 0.1 1 10 100 1000 Test results - - + + + +
[0049] In the above table, "-" means that the test result is negative, and "+" means that the test result is positive. From the test result, it can be drawn that the sensiti...
Embodiment 3
[0051] The colloidal gold immunochromatographic test paper for detecting tobacco mosaic virus prepared in Example 1 was used to detect field samples.
[0052] ① Sample extraction: take 0.1g (about 1cm) of tobacco leaves to be tested 2 ,), put it into the sample extraction bag (the extraction bag is a plastic bag with a grid in the middle interlayer, and the plastic bag is sealed with 4ml of 0.02M boric acid (pH=8.5) buffer), and mark the sample number; Or other smooth hard objects to rub the sample extraction bag to ensure that the tobacco leaves are completely ground, and shake the sample bag back and forth to mix the tobacco leaf extract evenly.
[0053] ② Detection method: Insert the sample pad end of the colloidal gold immunochromatographic test paper for detecting tobacco mosaic virus into the tobacco leaf extract to be tested. When the tobacco leaf extract is chromatographed to the middle of the test paper, take out the test paper, lay it flat, and react for 8 to 10 minu...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Sensitivity | aaaaa | aaaaa |
| Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More