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Method for repairing rare earth polluted soil by immobilized mixed fungicide

A mixed bacterial agent and rare earth pollution technology, which is applied in the field of repairing rare earth contaminated soil, can solve the problems of long growth cycle of super-accumulative plants, large changes in original soil, and removal of pollution sources, so as to prevent soil moisture from evaporating, low production costs, and environmental protection. small effect

Inactive Publication Date: 2014-07-16
INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different restoration technologies have their own advantages and disadvantages. The physical restoration method has the shortest period, the fastest effect, and the most thorough restoration, but the original soil changes greatly, requiring a large amount of engineering and high cost; chemical restoration has obvious effects and can maintain However, chemical remediation cannot remove pollution sources from the soil, and the use of chemical reagents may cause secondary pollution to the environment; phytoremediation is relatively low-carbon, environmentally friendly, and environmentally friendly, and it is not harmful to the original site. Cause damage, but hyperaccumulative plants have a long growth cycle and require multiple crops; as an emerging repair method, microorganisms are characterized by low cost and environmental friendliness, but the survival and growth of microorganisms are greatly affected by the environment, and are limited in the application process

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] (1) Preparation of strain mother liquor

[0036] Medium (g / L): Glucose 10.0 g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 3g, CaSO 4 2H 2 O 0.2 g, CaCO 3 5.0 g, beef extract 3g, peptone 5g,

[0037] Deionized water 1000 mL, pH 7.2, sterilized at 121 ℃ for 20 min

[0038] Enrichment medium containing rare earth ions: Add 1.5-2 ml of rare earth solution to the above medium to make the total concentration of rare earth ions 200-300 mg / L.

[0039] Inoculate the bacteria in the enrichment medium containing rare earth ions at a ratio of 2:1 by weight, add 10g of rare earth contaminated soil to the above medium, and cultivate in a constant temperature shaker at 28-30°C and 160 rpm / min for 5-7 God, wait for it.

[0040] (2) Preparation of primary seed solution

[0041] Inorganic medium: NaCl 1g, NH 4 SO 4 1g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.1g,

[0042] 1000 mL deionized water, pH 7.2, sterilized at 121 ℃ for 20 min

[0043] Inoculate the mother solutio...

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Abstract

The invention relates to a rare earth pollution repairing technology and particularly relates to an immobilized mixed fungicide for repairing rare earth polluted soil and a preparation method of the immobilized mixed fungicide. According to the method, bacillus megatherium and bacillus mucilaginosus are selected as the mixed fungicide for repairing rare earth polluted soil, the activity of the mixed fungicide can be kept for a long time by the immobilization technology, and the mixed fungicide has a high-efficiency performance stably in a complex environment. According to the technology, the bacillus megatherium and bacillus mucilaginosus not only can adsorb rare earth ions, but also can carry out potassium and phosphorus release and nitrogen fixation, and improve the fertility of soil; soil hardening can be prevented; soil moisture can be prevented from being evaporated. Crop byproducts are adopted as a carrier for immobilization, the sources are wide, the cost is low and the operation is simple. Not only can wastes be utilized, but also the crop byproducts can be used as a biofertilizer so as to provide various nutrients. Due to the technology, not only can be rare earth polluted soil be effectively repaired, but also the repaired soil is suitable for the growth of plants and the like.

Description

technical field [0001] The invention relates to a technology for repairing rare earth polluted soil, in particular to a technology and a preparation method for repairing rare earth polluted soil with an immobilized mixed bacterial agent. Background technique [0002] China is the country with the richest rare earth resources in the world, ranking first in the world in resource reserves, rare earth production, rare earth sales and rare earth consumption. In recent years, with the rapid development of science and technology and economy, especially the rapid development of various high-precision materials, the human demand for rare earth minerals is increasing, and the pace of development of rare earth minerals is gradually increasing. Only Baotou City In the past 10 years, the number of rare earth enterprises has increased by about 20. [0003] With the mining of rare earth deposits, a certain degree of rare earth pollution has been caused in rare earth mining and smelting ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12N11/02C12R1/11C12R1/07
Inventor 郑春丽张雪峰陈敏洁聂立王建英王亚琴程昊辰
Owner INNER MONGOLIA UNIV OF SCI & TECH
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