Method for repairing rare earth polluted soil by immobilized mixed fungicide
A mixed bacterial agent and rare earth pollution technology, which is applied in the field of repairing rare earth contaminated soil, can solve the problems of long growth cycle of super-accumulative plants, large changes in original soil, and removal of pollution sources, so as to prevent soil moisture from evaporating, low production costs, and environmental protection. small effect
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[0035] (1) Preparation of strain mother liquor
[0036] Medium (g / L): Glucose 10.0 g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 3g, CaSO 4 2H 2 O 0.2 g, CaCO 3 5.0 g, beef extract 3g, peptone 5g,
[0037] Deionized water 1000 mL, pH 7.2, sterilized at 121 ℃ for 20 min
[0038] Enrichment medium containing rare earth ions: Add 1.5-2 ml of rare earth solution to the above medium to make the total concentration of rare earth ions 200-300 mg / L.
[0039] Inoculate the bacteria in the enrichment medium containing rare earth ions at a ratio of 2:1 by weight, add 10g of rare earth contaminated soil to the above medium, and cultivate in a constant temperature shaker at 28-30°C and 160 rpm / min for 5-7 God, wait for it.
[0040] (2) Preparation of primary seed solution
[0041] Inorganic medium: NaCl 1g, NH 4 SO 4 1g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.1g,
[0042] 1000 mL deionized water, pH 7.2, sterilized at 121 ℃ for 20 min
[0043] Inoculate the mother solutio...
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