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A method for separating ethyl p-hydroxycinnamate from raspberry leaves

A technology of ethyl cinnamate and raspberry leaves, which is applied in the field of medicine and can solve problems such as no reports of compounds

Active Publication Date: 2015-08-05
曹庸
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the compound that inhibits intestinal peristalsis in raspberry leaves

Method used

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  • A method for separating ethyl p-hydroxycinnamate from raspberry leaves
  • A method for separating ethyl p-hydroxycinnamate from raspberry leaves

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Experimental program
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Effect test

Embodiment 1

[0018] Embodiment 1 prepares the obtained compound of the present invention and examines its activity

[0019] 1. Preparation of raspberry leaf extract

[0020] 5 Kg of raspberry leaves, a Chinese herbal medicine, were heated and rotated in a 60°C water bath with 85% ethanol for three times. After 2 hours, the extracts were combined, concentrated in vacuo to recover the solvent, and the extract extract was obtained;

[0021] 2. Screening of active sites that inhibit intestinal peristalsis

[0022] Dissolve the extract in water, extract with petroleum ether, chloroform, ethyl acetate and n-butanol in sequence, add 1 / 5 volume of extractant to the dissolved liquid each time, and extract about 15-20 times until the color of the extractant is close to zero. up to color. The extracts of petroleum ether, chloroform, ethyl acetate and n-butanol were respectively obtained, the solvents were recovered under reduced pressure, and the powders of each extraction part were ob...

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PUM

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Abstract

The invention provides a method for separating P-hydroxyl ethyl cinnamate from raspberry leaves. The method for separating the P-hydroxyl ethyl cinnamate from the raspberry leaves comprises the following steps: heating, rotating and extracting raspberry leaves for 3-5 times with ethyl alcohol water bath at the temperature of 60 DEG C, putting extracting solutions together, and carrying out vacuum concentration, so that extract is obtained; adding water into the extract and dissolving, extracting with ethyl acetate, carrying out silica gel column chromatography separation on extract liquor, carrying out medium pressure column chromatography separation firstly, wherein petroleum ether and ethyl acetate are adopted as eluant, carrying out gradient eluting by adopting the ethyl acetate and methyl alcohol, carrying out a domestic rabbit in vitro bowel movement experiment, and screening out components with strong anti-diarrhea activity; carrying out low pressure silica gel column chromatography separation purification on the components, carrying out gradient eluting on chloroform and methyl alcohol, putting the same components together, then carrying out column chromatography separation on the component, screened out through the domestic rabbit in vitro bowel movement experiment, with the strongest inhibiting effect by adopting an inverse ODS (ozone depleting substance) filler open column, and eluting with methyl alcohol-water, so that the P-hydroxyl ethyl cinnamate compound is obtained. The P-hydroxyl ethyl cinnamate compound is obtained from the raspberry leaves for the first time, has intestinal peristalsis resisting and anti-diarrhea effect and can be used for preparing a new natural anti-diarrhea medicine.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for separating ethyl p-hydroxycinnamate, a compound of raspberry leaves that has the functions of inhibiting intestinal peristalsis and anti-diarrhea, and the application of the compound. Background technique [0002] Raspberry (Rubus corchorifolius L.f) is a plant of the genus Rubus in the family Rosaceae. It is distributed all over the country except Northeast my country, Gansu, Xinjiang and Tibet, and has a large reserve of wild resources. Raspberry is a traditional Chinese herbal medicine long-term used by ethnic minorities in western Hunan and northwestern Hubei. People in western Hunan often mash the young leaves and feed them to animals to treat diarrhea. The effect is good. It is a safe and effective anti-diarrhea recipe recognized by the people. The previous research by Zhu Shenhai and our laboratory showed that raspberry leaf extract had inhibitory effect on t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/48A61P1/12
CPCC07C67/48C07C69/732
Inventor 常云佩刘晓娟曹庸刘欣周丽萍
Owner 曹庸
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