Cyanobacteria cell crushing method and device

A crushing method and technology of cyanobacteria, applied in the direction of microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of unsatisfactory cyanobacteria cell crushing effect, low cell crushing rate, time-consuming, etc., and achieve improvement Research and development efficiency and cyanobacteria natural product production efficiency, the effect of efficient methods

Inactive Publication Date: 2014-07-16
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no matter which method is used above, there are problems such as time-consuming, laborious and low crushing efficiency. For example, it usually takes several hours, and the cell crushing rate is less than 80%.
Although the glass bead grinding method is relatively simple, the crushing effect of the cyanobacteria cells is not ideal because the hardness of the glass beads is equivalent to that of cyanobacteria with a complex cell wall structure and the mechanical friction to the cyanobacteria cells is not ideal. extend

Method used

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  • Cyanobacteria cell crushing method and device
  • Cyanobacteria cell crushing method and device
  • Cyanobacteria cell crushing method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1, the device of emery grinding method broken cyanobacteria cell

[0055] The emery grinding method involved in the present invention breaks the device of cyanobacteria cell, can be any one of following (I)-(III) ( figure 1 ):

[0056] (1) A device consisting of a 1.5mL centrifuge tube and a matching grinding rod 1.

[0057] (II) A device consisting of a 50mL conical bottom centrifuge tube and a matching grinding rod 2 .

[0058] (III) A device consisting of a 15mL conical centrifuge tube and matching grinding rods 3 .

[0059] Wherein, the 1.5mL centrifuge tube is a product of Sangon Bioengineering Co., Ltd., and its catalog number is ST620-1; the 50mL conical bottom centrifuge tube is a product of Axygen Company, and its catalog number is SCT-50ML-25-S The 15mL conical bottom centrifuge tube is a product of Axygen Company, and its catalog number is SCT-15ML-25-S. The grinding rod 1 is a product of KimbleChase Company in the United States, and its catalo...

Embodiment 2

[0062] Embodiment 2, carborundum grinding method is used for crushing a small amount of freshwater single-cell cyanobacteria Synechocystis 6803

[0063] In this example, the freshwater unicellular cyanobacterium Synechocystis 6803 was crushed in a small amount by using the device in (I) of Example 1. details as follows:

[0064] 1. Preparation of algae liquid of freshwater unicellular cyanobacterium Synechocystis 6803

[0065] Scrape multiple algae colonies from the plate of freshwater cyanobacterium Synechocystis PCC6803 and inoculate them into BG-11 liquid medium; -2 .s -1 cultured to logarithmic phase (OD 730 For 3), standby.

[0066] 2. Emery grinding method to crush the freshwater single-celled cyanobacteria Synechocystis 6803

[0067] Take 1ml of the freshwater unicellular cyanobacterium Synechocystis 6803 obtained in step 1, add it to a 1.5ml centrifuge tube, centrifuge at 12000rpm (equivalent to 13000g), 4°C for 2min, discard the supernatant, collect the cells, a...

Embodiment 3

[0100] Embodiment 3, emery grinding method is used for crushing a small amount of seawater single-cell cyanobacterium Synechococcus 7002

[0101] In the present embodiment, adopt the device in (1) among the embodiment 1 to carry out a small amount of crushing to seawater unicellular cyanobacterium Synechococcus 7002. details as follows:

[0102] 1. Preparation of seawater unicellular cyanobacterium Synechococcus 7002 algae liquid

[0103] Referring to step 1 of Example 2, replace the freshwater unicellular cyanobacteria Synechocystis 6803 with the seawater unicellular cyanobacterium Synechococcus 7002.

[0104] 2. Emery grinding method to crush single-cell cyanobacteria Synechococcus 7002 in seawater

[0105] Refer to step 2 of Example 2, and replace the freshwater unicellular cyanobacteria Synechocystis 6803 with the seawater unicellular cyanobacterium Synechococcus 7002.

[0106] 3. Detection of the crushing effect of single-celled cyanobacteria Synechococcus 7002 in sea...

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Abstract

The invention discloses a cyanobacteria cell crushing method and a cyanobacteria cell crushing device. The cyanobacteria cell crushing method comprises the following steps: (1) centrifuging an alga liquid of cyanobacteria cells to be crushed, and discarding the supernate to obtain precipitate; and (2) adding emery into the precipitate obtained in the step (1). The experiment shows that the cyanobacteria cell crushing method disclosed by the invention is efficient and practical, is not only applicable to single cell cyanophyta, but also applicable to filiform cyanophyta, the time for crushing the cyanobacteria cells in a laboratory is shortened to be six minutes from hours, the cell crushing rate is increased to be more than 95% from the original 65%, and moreover later product extraction and product activity are not affected. Due to adoption of the method, difficulty of cell crushing in the fields of laboratory investigation and industrial application of cyanophyta is solved, and the cyanophyta research and development efficiency and the production efficiency of natural cyanophyta products are greatly improved.

Description

technical field [0001] The invention relates to a cyanobacteria cell crushing method and device. Background technique [0002] Blue-green algae, also known as cyanobacteria, is a prokaryotic organism that can perform oxygen-evolving photosynthesis. Cyanobacteria are widely distributed in fresh water, sea water and even sewage, and can utilize CO 2 and solar rapid reproduction, is the use of CO 2 An ideal host for biosynthetic chemicals, becoming a CO 2 Research hotspots in the field of emission reduction and renewable energy, such as the use of CO in cyanobacteria 2 Biosynthesis of chemicals such as alcohols, biodiesel and important proteins. In addition, most cyanobacteria are rich in nutrients and rich in important natural products, such as carotenoids, cyanobacteria proteins and anticancer drugs. Therefore, whether cyanobacteria is used as a host for the production of important chemicals or its natural products are developed; whether it is used for small-scale DNA an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12M1/00C12R1/89
Inventor 周杰张福良蒙恒凯李寅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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