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A Aspergillus oryzae strain producing β-fructosidase and its cultivation method and application

A kind of technology of Aspergillus oryzae and strain, applied in the field of microorganisms

Active Publication Date: 2017-10-17
SHANDONG UNIV
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Problems solved by technology

[0004] Known Aspergillus oryzae-derived β-fructosidase, only the report of inulin-type fructooligosaccharide synthesis, so looking for a new Aspergillus oryzae strain that can produce β-fructosidase of levan type fructooligosaccharide, to enzymatic method The synthesis of fructooligosaccharides is of great significance

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  • A Aspergillus oryzae strain producing β-fructosidase and its cultivation method and application
  • A Aspergillus oryzae strain producing β-fructosidase and its cultivation method and application

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Embodiment Construction

[0007] A strain of Aspergillus oryzae (Aspergillus oryzae) FS4 was preserved on April 24, 2014 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, the preservation number is CGMCC No.9087, address: No. 1, Beichen West Road, Chaoyang District, Beijing 3 Institute of Microbiology, Chinese Academy of Sciences.

[0008] Described Aspergillus oryzae (Aspergillus oryzae) FS4 bacterial strain grows rapidly on the potato culture medium, 2~3 days bacterium colony is spread out, produces a large amount of mycelia, and bacterium colony is white at the beginning, part yellow, becomes green when about 7 days, with the growth of culture time The elongation gradually turns brown to dark brown, and the hyphae in the base produce water-soluble brown pigment. Conidia heads radial, conidiophores subspherical or flask-shaped, covered with double-layered stalks. Conidia are pear-shaped or rounded when young, and spherical or subspherical when mature...

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Abstract

The invention relates to an Aspergillus oryzae strain producing β-fructosidase, a culture method and application thereof. A strain of Aspergillus oryzae (Aspergillus oryzae) FS4 was preserved on April 24, 2014 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, the preservation number is CGMCC No.9087, address: No. 1, Beichen West Road, Chaoyang District, Beijing 3 Institute of Microbiology, Chinese Academy of Sciences. The invention also relates to the cultivation method and application of the Aspergillus oryzae strain. The Aspergillus oryzae FS4 strain obtained by screening in the present invention is simple to cultivate, has stable genetic properties, high synthesis efficiency, and stable enzyme activity, and can be applied to the fields of modern bioengineering technology for enzymatically synthesizing levan-type fructo-oligosaccharides and the like, and has wide applications prospect.

Description

technical field [0001] The present invention relates to an Aspergillus oryzae strain producing β-fructosidase and its cultivation method and application, in particular to an Aspergillus oryzae strain capable of producing β-fructosidase (EC3.2.1.26) and its cultivation method and its application in The invention is applied in synthesizing levan type fructo-oligosaccharides and belongs to the technical field of microorganisms. technical background [0002] Fructo-oligosaccharides are a general term for fructooligosaccharides in which fructosyl groups are linked to sucrose (GF) molecules by β (2-1) bonds (inulin type) and β (2-6) bonds (levan type). Fructo-oligosaccharide is a new type of sweetener with health functions such as regulating intestinal flora, proliferating bifidobacteria, promoting calcium absorption, regulating blood lipids, and anti-caries. At present, the production of fructooligosaccharides is mainly divided into plant extraction method and enzymatic method. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/24C12P19/14C12R1/69
Inventor 肖敏徐莉卢丽丽宋德勇王冬雪
Owner SHANDONG UNIV