Preparation method and application of papaya effective composition

An effective component and papaya technology, which is applied in the field of preparation of papaya effective components, can solve the problems of the preparation method and activity report of papaya effective components, etc., and achieve the effects of scavenging free radicals and delaying skin aging.

Active Publication Date: 2014-07-30
H&B COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the preparation method and activity of the effective components of papaya in the prior art.

Method used

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  • Preparation method and application of papaya effective composition
  • Preparation method and application of papaya effective composition
  • Preparation method and application of papaya effective composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Get papaya papaya, extract 3 times with ethanol with a mass percentage concentration of 85%, each extraction time is 2 hours, the weight of ethanol added during each extraction is 10 times the weight of papaya papaya, and the extracts are combined for 3 times to obtain extraction liquid; concentrate the extract until it has no alcohol smell, add 10 times the weight of papaya papaya to dilute with water, filter it with a 300-mesh sieve, and then add it to HPD100 macroporous adsorption resin for adsorption. After the adsorption is completed, wash it with water until it flows out The solution is colorless, and then eluted with 80% ethanol aqueous solution to obtain an eluate, and then the eluate is concentrated to obtain the effective component of papaya.

[0023] Among them, take 0.2ml of eluent, add 0.2ml of 5% vanillin-glacial acetic acid solution, shake well, and then add 1.0ml of perchloric acid solution. Heat in a water bath at 60°C for 5 minutes. Shake well and obs...

Embodiment 2

[0026] Get the papaya papaya, extract 4 times with ethanol with a concentration of 80% in mass percentage, and the time of each extraction is 0.5 hour, and the weight of adding ethanol during each extraction is 5 times of the weight of papaya papaya, merge to obtain the extract; Concentrate the liquid until it has no alcohol smell, add 10 times the weight of papaya papaya to dilute with water, filter it with a 300-mesh mesh, and then add it to HPD100 macroporous adsorption resin for adsorption. After the adsorption is completed, wash it with water until the effluent is colorless , and then eluted with an aqueous ethanol solution with a mass percentage concentration of 80% to obtain an eluent, and then concentrate the eluent to obtain the effective components of papaya.

[0027] Among them, take 0.2ml of eluent, add 0.2ml of 5% vanillin-glacial acetic acid solution, shake well, and then add 1.0ml of perchloric acid solution. Heat in a water bath at 60°C for 5 minutes. Shake we...

Embodiment 3

[0029] Get wrinkled papaya, extract once with ethanol with a mass percentage concentration of 90%, and the time of each extraction is 4 hours. The weight of ethanol added during each extraction is 20 times the weight of wrinkled papaya to obtain an extract; The liquid is concentrated until it has no alcohol smell, diluted with water 10 times the weight of wrinkled papaya, filtered through a 300-mesh sieve, and then added to HPD100 macroporous adsorption resin for adsorption. After the adsorption is completed, it is washed with water until the effluent is free. color, and then eluted with 80% ethanol aqueous solution to obtain the eluent, and then concentrated the eluent to obtain the effective components of papaya.

[0030] Among them, take 0.2ml of eluent, add 0.2ml of 5% vanillin-glacial acetic acid solution, shake well, and then add 1.0ml of perchloric acid solution. Heat in a water bath at 60°C for 5 minutes. Shake well and observe with the naked eye until the eluent samp...

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Abstract

The invention relates to a preparation method and an application of a papaya effective composition, and belongs to the field of traditional Chinese medicine active composition extraction. The preparation process includes the following steps: taking papaya, and extracting with ethanol to obtain an extracted liquid; concentrating the extracted liquid until no alcohol smell exist, diluting by adding water, filtering, then adding onto an HPD100 macropore adsorption resin, and adsorbing; after completion of adsorption, eluting with water until the effluent is colorless; then eluting with an ethanol aqueous solution with the mass percentage concentration of 80% to obtain an eluate; and followed by concentrating the eluate, and thus obtaining the papaya effective composition. The method has the beneficial effects that the papaya effective composition obtained by the preparation method is rich in triterpenoid and other effective components, and has the activities of resisting oxidation, resisting aging, eliminating free radicals, resisting mutation, delaying skin aging and the like.

Description

technical field [0001] The invention belongs to the field of extraction of effective components of traditional Chinese medicines, and in particular relates to a preparation method and application of effective components of papaya. Background technique [0002] Papaya is the dry and near-ripe fruit of Chaenomeles speciosa (Sweet) Nakai, a plant of the Rosaceae family. It has been included in the "Pharmacopoeia of the People's Republic of China" (2010 edition), and it is rich in chemical components such as saponins, flavonoids, polysaccharides, organic acids, and tannins. It has the functions of calming the liver and relaxing the tendons, relieving the stomach and eliminating dampness. For arthralgia due to dampness spasm, soreness and pain in the waist and knee joints, vomiting, diarrhea and tendons turning, beriberi edema. It is mainly produced in Anhui, Zhejiang, Henan, Hubei and Sichuan in my country. Among them, Anhui Xuancheng papaya, Hubei Ziqiu papaya and Zhejiang C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K36/732A61Q19/08A61P39/06A61P17/18A23L1/29A23L33/00
Inventor 黄灿许刚杨立新赵都宁
Owner H&B COSMETICS
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