Universal method for preparing polymeric vesicles with sensitive response

Abstract

The invention relates to an amphiphilic block polymer, polymeric vesicles formed by the amphiphilic block polymer and a preparation method and application thereof. More specifically, the amphiphilic block polymer is prepared from a hydrophobic chain segment and a hydrophilic chain segment by a polyurethane polycondensation and reversible additive fragment transfer (RAFT) polymerization method, wherein the hydrophobic chain segment is a chain transfer agent containing programmed-degraded polymer represented by a following formula shown in a drawing, and the hydrophilic chain segment is hydrophilic poly(N,N-dimethylacrylamide) and has the molecular weight of 2,000-15,000Da. Through using the amphiphilic block polymer, the polymeric vesicles have the advantages that the controlled synchronous release of hydrophilic and hydrophobic molecules loaded by the vesicles is realized, and the biocatalysis activity of enzymes loaded by the vesicles is regulated and controlled by multiple stimulation programs.

Description

technical field [0001] The present invention relates to polymer materials and their applications, more specifically, to a stimulus-response-triggered self-degradable amphiphilic block polymer, polymer vesicles composed of the amphiphilic block polymer, and a preparation method thereof and apply. Background technique [0002] Liposome vesicles and polymer vesicles are artificial self-assembled nanostructures formed by a hydrophilic inner cavity wrapped by a hydrophobic bilayer membrane, and are ideal for humans to explore the structure and structure of complex biological systems such as cells and viruses. A typical example of a function. These vesicles are used to construct drug delivery nanocarriers, nanoreactors and artificial organelles (Wolfgang Meier, et al. Acc. Chem. Res. 2011, 44, 1039-1049). Obviously, all of these applications are closely related to the efficient exchange of substances in and out of the vesicle membrane. However, compared with liposomal vesicles,...

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Problems solved by technology

However, compared with liposomal vesicles, polymer vesicles with a more stable structure face serious membrane permeability problems (Adi Eisenberg, et al. Science 2002, 297, 967-973), such as small organic molecules, ions and even water is difficult to penetrate

To solve this problem, block copolymers containing nucleating blocks capable of changing solubility in response to external stimuli are used to construct polymersomes, but the change in polymer solubility requires the physicochemical properties of most of the units of the polymer chain. change, thus requiring high stimulus concentration and energy

Another strategy is to use degradable or easily cleavable polymers to prepare polymersomes. These vesicles can be lysed by spontaneous or random degradation, but vesicle cleavage still requires a lot of stimulation time and its degradation characteristics Uncontrollable, in addition, its degraded intermediates aggregate due to lack of water solubility

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