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A kind of cultivation method of broccoli sprout rich in sulforaphane

A technology of sulforaphane and cultivation method, applied in the directions of botanical equipment and methods, cultivation, germination equipment, etc., can solve the problems of less research, low sulforaphane, difficult industrialized production, etc., to prevent erosion and improve germination rate Effect

Inactive Publication Date: 2015-08-12
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of sulforaphane in broccoli is low, it is difficult to realize industrial production, and the current domestic research on broccoli mainly focuses on the storage and preservation of broccoli, variety selection, etc. There are few studies on the enrichment and strengthening of active substances, so finding a way to increase the content of sulforaphane in broccoli becomes the key to the further development and utilization of broccoli resources

Method used

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  • A kind of cultivation method of broccoli sprout rich in sulforaphane
  • A kind of cultivation method of broccoli sprout rich in sulforaphane
  • A kind of cultivation method of broccoli sprout rich in sulforaphane

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Effect test

Embodiment 1

[0035] A method for cultivating broccoli sprouts rich in sulforaphane, comprising the following specific steps:

[0036] 1. Selection and treatment of broccoli seeds

[0037] Select broccoli seeds that are harvested in the current year, have no mildew and damage, no impurities, and full granules, soak them in an aqueous solution of flavonoids with a mass concentration of 7-9% for 10 hours, and then rinse them with water for 3 times;

[0038] Wherein the preparation method of the crude flavonoids of cabbage is as follows: fresh cabbage is dried at 75°C for 30 hours, then pulverized with a pulverizer and passed through an 80-mesh sieve, and the powder of cabbage is mixed with 30% ethanol solution according to the ratio of solid to liquid of 1:50 Then extract at 55°C for 4.5 hours, then centrifuge the above extract at 3000r / min for 10min, take the supernatant and vacuum filter to obtain the extract of flavonoids, and finally freeze-dry the extract to obtain Crude flavonoids of c...

Embodiment 2

[0052] Basically with above-mentioned embodiment 1, its difference is:

[0053] 1) Selection and treatment of broccoli seeds: Soak the selected broccoli seeds in an aqueous solution of crude flavonoids with a concentration of 7% for 12 hours; dry fresh cabbage at 70°C for 36 hours, then pulverize them with a grinder and grind them. Pass through a 80-mesh sieve, then mix the cabbage powder with 30% ethanol solution and extract at 50°C for 5 hours;

[0054] 2) Germination of broccoli seeds: place the broccoli seeds in an environment of 25°C and 80% humidity for cultivation and germination until the broccoli seeds are white;

[0055] 3) Cultivation of sulforaphane-rich broccoli sprouts: Cultivate dewy broccoli seeds in an environment with a temperature of 20°C and a humidity of 80% until the broccoli sprouts grow to 8-10mm or the broccoli roots grow to 10-12mm. Harvest.

[0056] The content of sulforaphane in the broccoli sprouts cultivated by this implementation method is 5.86mg...

Embodiment 3

[0058] Basically with above-mentioned embodiment 1, its difference is:

[0059] 1) Selection and treatment of broccoli seeds: Soak the selected broccoli seeds in an aqueous solution of crude flavonoids with a concentration of 9% for 8 hours; dry the fresh cabbage at 80°C for 24 hours, then pulverize them with a grinder and grind them. Pass through a 80-mesh sieve, then mix the cabbage powder with 30% ethanol solution and extract at 60°C for 4 hours;

[0060] 2) Germination of broccoli seeds: place the broccoli seeds in an environment of 30°C and 90% humidity for cultivation and germination until the broccoli seeds are white;

[0061] 3) Cultivation of sulforaphane-rich broccoli sprouts: Cultivate dewy broccoli seeds in an environment with a temperature of 25°C and a humidity of 90% until the broccoli sprouts grow to 8-10mm or the broccoli roots grow to 10-12mm. Harvest.

[0062] The content of sulforaphane in the broccoli sprouts cultivated by this implementation method is 5...

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Abstract

The invention discloses a cultivation method for broccoli buds rich in sulforaphane. The cultivation method is characterized by comprising the steps of the extraction of sedum aizoon crude flavonoids, the preparation of a self-made culture solution, the selection and processing of broccoli seeds, the germination of the broccoli seeds, the reveal of the broccoli seeds, the cultivation of the broccoli seeds through using a mode that the revealed broccoli seeds are placed on gauze which is wetted by the self-made culture solution under a certain light condition and an environment with the temperature of 20-25 DEG C and the humidity of 80-90 %, the spraying of the self-made culture solution every other 24 hours, and the harvesting when the broccoli buds grow to 8-10 mm or the broccoli roots are 10-12 mm long to obtain the broccoli buds rich in the sulforaphane. The cultivation method has the advantages that the resistance to corrosion is strong, the yield is high, the content of the sulforaphane is high, the cultivation method is simple, and the effect is rapid.

Description

technical field [0001] The invention relates to a method for cultivating broccoli sprouts, in particular to a method for cultivating broccoli sprouts rich in sulforaphane. Background technique [0002] Sulforaphane is a multifunctional enzyme inducer, which can induce the body to produce type II detoxification enzymes-glutathione transferase (GST) and quinone reductase (QR), which can neutralize suspected carcinogens and prevent carcinogens Destroys genetic factors in healthy cells, thus playing an anti-cancer role. [0003] Broccoli, also known as green cauliflower, broccoli, tender stem cauliflower, etc., is a variety of Brassica Brassica Brassica species. It is a one-year or two-year herbaceous plant. It is native to Italy and was introduced to China in the early 19th century. The nutritional components in broccoli are not only high in content, but also very comprehensive. The content of sulforaphane in it ranks first among all vegetables. The results of a research on an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01C1/02
Inventor 许凤王鸿飞董迪迪邵兴锋李和生汤月昌
Owner NINGBO UNIV
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