Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of SSR primer and method for the purity identification of Linglong zucchini hybrid seeds

A technology for the purity and identification method of hybrid seeds, which is applied in the field of identification of hybrid seed purity, can solve problems such as the lack of establishment of methods for identification of the purity of zucchini hybrid seeds, and achieve high commercial application value, simple operation, and strong specificity

Active Publication Date: 2016-01-20
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the prior art, a method for identifying the purity of Zygodon hybrid seeds has not yet been established.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of SSR primer and method for the purity identification of Linglong zucchini hybrid seeds
  • A kind of SSR primer and method for the purity identification of Linglong zucchini hybrid seeds
  • A kind of SSR primer and method for the purity identification of Linglong zucchini hybrid seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Establishment of the method for detecting the purity of hybrid Linglong zucchini seeds

[0029] 1. Screen the SSR primers for purity identification

[0030] Several pairs of primers designed according to the sequencing results of the wax gourd transcriptome were screened between the parents (Jiangxinjie No. 6 of the male parent and Qiang Female Line No. 4 of the female parent) and offspring, and the primer ChQSSR304 of the co-dominant differential marker band was selected, The marking band is clear and repeatable, and the sequence is as follows:

[0031] ChQSSR304-F:5'-GGGGCCTGAAAATGAAGTAA-3' (SEQ ID NO: 1)

[0032] ChQSSR304-R: 5'-AAAACATGGTCTAGGGCTATGC-3' (SEQ ID NO: 2).

[0033] Using Zucchini genomic DNA as a template, the above SSR primer ChQSSR304 was used for PCR amplification, and the amplified product was subjected to gel electrophoresis. The electrophoresis results showed that the SSR primer ChQSSR304 can produce a 185bp paternal specific marker...

Embodiment 2

[0057] Adopt the method of embodiment 1 to 100 strains of Linglong zucchini that get from the seed purity identification field of Dafeng base, number its single plant, extract single plant DNA and detect (see figure 2 ), test results: 90 hybrid seeds, 10 female parents, the seed purity is 90%, consistent with the field test results.

Embodiment 3

[0059] Adopt the method of embodiment 1 to 100 strains of Linglong zucchini that are taken from the seed purity identification field of Vegetable Institute base, number its single plant, extract single plant DNA and detect (see image 3 ), the test results are all true hybrids, the seed purity is 100%, which is consistent with the field test results, and the accuracy rate is 100%.

[0060] The above examples show that the method of the present invention can effectively distinguish the hybrid seeds of Linglong Zucchini from its female parent (Qiangyo No. 4), and quickly and accurately detect the purity of the seeds.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an SSR primer and a method for the purity identification of Linglong zucchini hybrid seeds. The primer includes an upstream primer ChQSSR304-F and a downstream primer of ChQSSR304-R of a primer ChQSSR304. The upstream primer ChQSSR304-F has a nucleotide sequence shown as SEQ ID NO.1, and the downstream primer ChQSSR304-R has a nucleotide sequence shown as SEQ ID NO.2. The invention also discloses a method for purity identification of Linglong zucchini hybrid seeds. The primer and method can effectively distinguish the Linglong zucchini hybrid seeds and female parent and male parent seeds thereof, fast and accurately detect purity of hybrid seeds, and has the advantages of rapidness, accuracy, low cost and simple operation, etc.

Description

technical field [0001] The invention belongs to the technical field of purity identification of hybrid seeds, and in particular relates to an SSR primer for identifying the purity of Linglong zucchini hybrid seeds and a method for identifying the purity of Linglong zucchini hybrid seeds. Background technique [0002] Seed purity is one of the most important quality indicators for seed quality inspection. The test of Distinctness, Uniformity and Stability of new plant varieties (DSU test for short) is the most important work content in the substantive examination of the new variety rights granted by the Plant New Variety Protection Office of the Ministry of Agriculture. . [0003] Linglong zucchini is a hybrid first-generation zucchini variety. When producing seeds, the parents (male parent and female parent) breeding field, the first-generation hybrid production field and other varieties or types of zucchini and wax gourd materials must be strictly separated, and the separ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 何晓明彭庆务郭明鑫谢大森江彪罗少波黄智文刘文睿林毓娥刘洪彪
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI