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Method for extracting fish scale collagen by using energy-gathered type pulsed ultrasound assistant hot water

A fish scale collagen and hot water extraction technology, applied in chemical instruments and methods, animal/human proteins, connective tissue peptides, etc., can solve the problems of high extraction cost, low extraction rate, and short extraction time by enzymatic extraction. Achieve the effect of simple and easy processing method, improvement of extraction rate and reduction of production cost

Inactive Publication Date: 2014-08-27
江苏江大五棵松生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the help of ultrasound to strengthen the hot water extraction effect of fish scale collagen, a new extraction method is established by using the mechanical effect, thermal effect and cavitation effect produced by ultrasonic waves on the medium, which can improve the low extraction rate of the traditional hot water extraction method. At the same time, there will be no problems such as collagen degradation caused by alkali extraction and high extraction cost by enzyme extraction. It has the characteristics of short extraction time, high extraction rate, less energy consumption, and structural integrity.

Method used

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  • Method for extracting fish scale collagen by using energy-gathered type pulsed ultrasound assistant hot water
  • Method for extracting fish scale collagen by using energy-gathered type pulsed ultrasound assistant hot water

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Weigh 500g of fish scales, crush them to 40 mesh, soak the fish scales in 10% (w / v) NaCl solution for 24 hours, change the liquid once in the middle, remove the impurities and fish silver powder attached to the surface of the fish scales, and use hydrochloric acid to remove the minerals in the fish scales. substance, the acid concentration is 0.5mol / L, the liquid-solid ratio is 20mL / g, and the deashing time is 80min. Weigh 80 g of delimed fish scales, add 50 times of water to dissolve, heat the feed solution to 70° C., and conduct focused pulsed ultrasonic-assisted extraction of fish scale collagen. Ultrasonic-assisted extraction is carried out by applying ultrasonic waves intermittently. The extraction conditions are as follows: extraction time 50 minutes, extraction liquid pH 3, pulse ultrasonic sounding time 10 s and intermittent time 5 s, material liquid passes through the probe in a countercurrent circulation, and the pump speed flux is 180 mL / min, the ultrasonic ...

Embodiment 2

[0023] Weigh 500g of fish scales, crush them to 40 mesh, soak the fish scales in 10% (w / v) NaCl solution for 24 hours, change the liquid once in the middle, remove the impurities and fish silver powder attached to the surface of the fish scales, and use hydrochloric acid to remove the minerals in the fish scales. substance, the acid concentration is 0.5mol / L, the liquid-solid ratio is 20mL / g, and the deashing time is 80min. Weigh 80g of delimed fish scales, add 40 times of water to dissolve, heat the feed solution to 50°C, and perform concentrated pulsed ultrasonic-assisted extraction of fish scale collagen. Ultrasonic-assisted extraction is carried out by applying ultrasonic waves intermittently. The extraction conditions are as follows: extraction time 50 minutes, extraction liquid pH 3, pulse ultrasonic sounding time 10 s and intermittent time 5 s, material liquid passes through the probe in a countercurrent circulation, and the pump speed flux is 180 mL / min, the ultrasoni...

Embodiment 3

[0025] The test process is the same as that in Example 1, except that 80 g of delimed fish scales are weighed, dissolved in 40 times of water, and the feed solution is heated to 70° C. to perform focused pulsed ultrasonic-assisted extraction of fish scale collagen. Ultrasonic-assisted extraction is carried out by applying ultrasonic waves intermittently. The extraction conditions are as follows: extraction time 50 minutes, extraction liquid pH 6, pulse ultrasonic sounding time 10 s and intermittent time 5 s, material liquid passes through the probe in a countercurrent circulation, and the pump speed flux is 180 mL / min, the ultrasonic power is 150w, the frequency is 28kHz, and the extraction rate of collagen is 79.55%.

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Abstract

The invention provides a method for extracting fish scale collagen by using energy-gathered type pulsed ultrasound assistant hot water, and belongs to the field of deep development and utilization of fishery products. The method comprises the steps of weighing 500 g of fish scales; smashing the fish scales; removing impurities preliminarily; removing mineral substances from the fish scales by using hydrochloric acid under the conditions that an acid concentration is 0.2-0.8 mol / L, a removal time is 20-100 min, and a liquid-solid ratio is 10-35 mL / g; adding 30-60 times water to decalcified fish scales to dissolve the fish scales; and extracting the collagen by using energy-gathered type pulsed ultrasound assistant hot water under the extraction conditions that a liquid temperature is 40-80 DEG C; an extraction time is 20-70 min; an extraction pH is 3-8; and ultrasonic parameters are as follows: a frequency is 28 kHz; a power is 150W; a pulse sending time is 4-12 s and a pulse intermittent time is 2-6 s. The method is simple and practicable, is convenient for operation and can be applied in continuously large-scale production.

Description

technical field [0001] The invention relates to the field of development and utilization of fishery processing by-products, in particular to the production of health food, in particular to a method for extracting collagen from fish scales assisted by hot water with energy-gathering pulse ultrasound. Background technique [0002] Collagen is a fibrous protein found in nearly every tissue in the body, including skin, blood vessels, tendons and teeth, as well as blood vessels and intestines. Collagen from fish scales is widely used in medical products, cosmetics, food and other industries. [0003] Fish scale is the deformation of the dermis of fish skin, which is rich in protein, mainly collagen and scale keratin, with a salt content of 30% to 46%. According to statistics, the total amount of fish by-products in my country is more than 2.5 million tons every year, of which fish scales account for about 15%, that is, the annual output of fish scales can reach more than 350,000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14
CPCC07K14/78
Inventor 马海乐郝俊杰王振斌周存山
Owner 江苏江大五棵松生物科技有限公司
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