A method for detection of complete gene deletion of cyp2a6 based on fluorescent quantitative PCR

Abstract

The invention provides a method for detecting CYP2A6 whole-gene deletion based on a fluorescent quantitative PCR. The method is used for achieving the aim of non-disease diagnosis. Highly-specific primers for CYP2A6 genes and ALB genes are respectively designed, and CYP2A6 whole-gene deletion is detected fast and accurately based on the fluorescent quantitative PCR technology. Primer probe combinations for CPY2A6 comprise Fp:5'-TCCCACCCTACTCCCTCTCTC-3', Rp:5'-GGAGGTGAACGCGGGA-3' and Probe:5'-FAM-TGGAGACAGGCCAGGGGGCG-BHQ2-3'.

Description

technical field [0001] The invention relates to a method for detecting CYP2A6 whole gene deletion. Background technique [0002] The CYP2A6 gene is a member of the human cytochrome P450 gene superfamily, and the protein encoded by it is an important metabolic enzyme involved in the metabolism of various drugs and toxic compounds in the human body. The CYP2A6 gene shows a wide range of polymorphisms in the population, and the frequency of CYP2A6 gene deletion in the Chinese population is 5%-15%. [0003] Tegafur and compounds with tegafur as the main active ingredient are currently the most widely used anti-pyrimidine drugs in clinical practice, and have good therapeutic effects on digestive tract tumors and other solid tumors. [0004] A large number of studies have shown that the CYP2A6 gene deletion polymorphism is closely related to the efficacy of drugs such as S-1 and UFT with tegafur as the active ingredient. Patients without the polymorphism had higher drug response...

AI Technical Summary

Problems solved by technology

[0007] However, this technology has not been applied to the detection of CYP2A6 gene deletion; the fundamental reason is that the CYP2A6 gene has a very high sequence homology (more than 90%) with the CYP2A7 gene and CYP2A13 gene. It is very difficult to combine primers and probes to amplify CYP2A6 gene with high specificity in PCR reaction

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