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Preparation method for targeting contrast agent for intestinal tracts

A technology for targeting contrast agents and intestines, applied in the field of nano-biomedical materials, can solve problems such as non-targeting, and achieve the effects of avoiding poor targeting, satisfying production and application, and high biological safety.

Inactive Publication Date: 2014-09-03
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the contrast agents in clinical application are non-specific extracellular fluid space contrast agents without targeting

Method used

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  • Preparation method for targeting contrast agent for intestinal tracts
  • Preparation method for targeting contrast agent for intestinal tracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Synthesis of Fe3O4 nanoparticles modified by dicarboxypolyethylene glycol:

[0024] 2.1g Fe(acac) 3 , 7.9ml oleylamine and 24g dicarboxy polyethylene glycol HOOC-PEG-COOH (molecular weight 2000) were dissolved in 200ml diphenyl ether. The solution was stirred at 400 rpm for 2 hours under nitrogen to remove oxygen. After incubation at 80°C for 4 hours, the solution was quickly boiled for more than 30 minutes. After the solution was cooled, it was washed three times with ether and ethanol respectively. Then add PBS buffer to disperse evenly.

[0025] 2. Antibody modification:

[0026] Dissolve 2 mg of the above iron ferric oxide particles in 0.01 M PBS buffer solution, add 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC, 2.5 mM) and N-hydroxy Succinimide (NHS, 6.25 mM), after about 15 minutes, 10 mL of TLR4 antibody (0.1 mg / ml) was added. React at 4 degrees Celsius for more than 10 hours, then centrifuge at 12,000 rpm to remove unreacted antibodies, and re-disp...

Embodiment 2

[0028] 1. Synthesis of Fe3O4 nanoparticles modified with carboxyaminopolyethylene glycol:

[0029] 1.6g FeCl 3 ·6H 2 O, 8.5ml oleic acid and 24g carboxyaminopolyethylene glycol NH 2 - PEG-COOH (molecular weight 2000) was dissolved in 200ml of diphenyl ether. The solution was stirred at 400 rpm for 2 hours under nitrogen to remove oxygen. After incubation at 80°C for 4 hours, the solution was quickly boiled for more than 30 minutes. After the solution was cooled, it was washed three times with ether and ethanol respectively. Then add PBS buffer to disperse evenly.

[0030] 2. Antibody modification:

[0031] Dissolve 2 mg of the above iron ferric oxide particles in 0.01 M PBS buffer solution, add 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC, 2.5 mM) and N-hydroxy Succinimide (NHS, 6.25 mM), after about 15 minutes, 10 mL of TLR4 antibody (0.1 mg / ml) was added. React at 4 degrees Celsius for more than 10 hours, then centrifuge at 12,000 rpm to remove unreacted antibod...

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Abstract

A preparation method for a targeting contrast agent for intestinal tracts comprises synthesis of a ferriferrous oxide nanometer particle modified by a functional high molecule and modification of an antibody. The targeting contrast agent for intestinal tracts has the advantages that the nanometer ferriferrous oxide particle is taken as a medium and is connected with a specific antibody for forming the contrast agent for intestinal-tract targeting property, so that the problem that conventional contrast agents are bad in targeting property is avoided. Employed raw materials are high in biological safety, and parts of the raw materials are commercial products. The prepared contrast agent possesses good physical chemical stability, biological compatibility and magnetic resonance imaging performance. The preparation method is simple in technology and high in operationality, and is capable of further satisfying production and application requirements.

Description

technical field [0001] The invention relates to a method for preparing an intestinal-targeted magnetic resonance contrast agent, in particular to a contrast agent preparation technology that uses superparamagnetic iron oxide as a medium and simultaneously links antibodies on the surface of macrophage cell membranes in inflammatory bowel disease. The invention belongs to the field of nano biomedical materials. Background technique [0002] Inflammatory bowel disease (IBD) is a type of intestinal inflammatory disease whose etiology and pathogenesis are not yet fully understood. Magnetic resonance (MRI) colonoscopy can be used to detect and quantify ulcerative colitis, reflecting the degree of colonic lesions and activities. In contrast-enhanced MRI examination, the degree of enhancement of the intestinal wall is related to the degree of inflammatory activity. Magnetic resonance imaging can be freely selected in the process of image acquisition, and has the advantage of provi...

Claims

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Application Information

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IPC IPC(8): A61K49/18A61K49/12
Inventor 何丹农王萍朱君颜娟夏智伟
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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