Polypeptide based melanoma biomarker sensor
A biomarker and melanoma technology, applied in the field of melanoma biomarker sensors, can solve the problems of complex chemical structure, long production cycle, complicated production process, etc., and achieve simple chemical structure, simple and clear chemical structure, and detection sensitivity Improved effect
Inactive Publication Date: 2014-09-03
李根喜
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Problems solved by technology
Relying on antibodies to recognize target proteins has certain limitations, mainly reflected in: (1) As a protein, antibodies have complex chemical structures, so they cannot be achieved through artificial synthesis.
(2) The production of antibodies requires cell lines or animals, and the production process is complicated and the production cycle is long; (3) Antibodies are relatively expensive and unstable
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Embodiment Construction
[0016] Combine below Figure 1 to Figure 4 The preferred embodiments of the present invention are given to describe the technical solution of the present invention in detail.
[0017] Recognition peptide [k(MUA)-KRLRRSAHARKETEFLRLKRTRLGLE, lyophilized powder, purity>95%] and signal peptide [D(GHK)-KRLRRSAHARKETEFLRLKRTRLGLE, lyophilized powder, purity>95%] were ordered from Shanghai Keyeptide Biotechnology Company, recombinant human S100B protein (purity>95%) was purchased from Sino Biological Company, TCEP, MNH and BSA were purchased from Sigma Company, Spectra / Pro7 standard-grade regenerated cellulose dialysis membrane was purchased from Spectrum Company, and other reagents were analytically pure, without For additional processing, the recognition peptide and signal peptide lyophilized powder were dissolved in 20 mM Tris-Hcl (pH 7.5). The target protein S100B freeze-dried powder is dissolved in 20mM Tris-Hcl solution containing 1mM TCEP (pH7.5), and then formulated into dif...
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The invention discloses a polypeptide based melanoma biomarker sensor. According to the polypeptide based melanoma biomarker sensor, two specific peptides can be combined in a 1: 2 manner through a melanoma biomarker; the terminal of one polypeptide is modified with MUA, the polypeptide is fixed on the surface of an electrode, so as to identify the melanoma biomarker, and the terminal of the other polypeptide is modified with GHK, so as to output electrochemical signals; the two peptides are respectively named an identification peptide and a signal peptide; in the presence of the melanoma biomarker, the identification peptide can specifically identify and combine the melanoma biomarker, so as to form the polypeptide based melanoma biomarker sensor disclosed by the invention. The polypeptide based melanoma biomarker sensor has good sensitivity and specificity and has the advantages of simplicity, low consumption and the like.
Description
technical field [0001] The present invention relates to a sensor, in particular to a melanoma biomarker sensor based on polypeptide. Background technique [0002] As a very aggressive and high-mortality tumor, melanoma is characterized by unpredictability of metastasis and high risk of recurrence and death. Even after surgical treatment, the risk of recurrence and death of patients still exists . Therefore, timely monitoring of tumor progression and recurrence is very important. Research results in recent years have shown that S100B (target protein) is an independent melanoma biomarker, which is not only closely related to tumor stage, disease progression and recurrence, but also provides prognosis information and predicts the prognosis of melanoma patients. corresponding therapeutic effects. Based on the unique biological significance of the melanoma biomarker S100B, there have been relevant literature reports: as a melanoma biomarker, S100B is significantly better than ...
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IPC IPC(8): G01N33/68
CPCG01N33/57488
Inventor 李根喜殷咏梅王晓盈仇金荣
Owner 李根喜