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Dehydrogenase electrode, and preparation method and application thereof

A dehydrogenase and electrode technology, applied in battery electrodes, circuits, electrical components, etc., can solve the problems of poor reversibility of electrochemical reactions, complicated preparation process, poor electronic conductivity, etc., and achieve high electrochemical reversibility and wide source of raw materials , The effect of simple preparation method

Active Publication Date: 2010-12-29
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, while the above method achieves the electrochemical activity of polyaniline in neutral solution, there are more or less disadvantages such as complicated preparation process, poor reversibility of electrochemical reaction, poor electronic conductivity, and difficulty in industrialization.

Method used

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  • Dehydrogenase electrode, and preparation method and application thereof
  • Dehydrogenase electrode, and preparation method and application thereof
  • Dehydrogenase electrode, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the preparation of polyaniline

[0024] Dissolve 186mg of aniline monomer and 456mg of potassium persulfate in 10mL of 0.5M hydrochloric acid solution, react in an ice bath at 0-5°C for 5 hours, filter the precipitate, wash with hydrochloric acid and double distilled water, and dry in vacuo to obtain green polymer Aniline (number average molecular weight 75400).

Embodiment 2

[0025] Embodiment 2, the preparation of polyaniline

[0026] Add 294mg of aniline monomer and 348mg of manganese dioxide into 20mL of 1M hydrochloric acid solution, react in an ice bath at 0-10°C for 10 hours, filter the precipitate, wash with hydrochloric acid and double distilled water, and dry in vacuum to obtain green polyaniline ( Number average molecular weight 32100).

Embodiment 3

[0027] Embodiment 3, polyaniline / carbon nanotube composite electrode

[0028] 1.5 mg of polyaniline prepared in Example 1 (number average molecular weight 75400) and 3 mg of carbon nanotubes (Shenzhen Nano Harbor Co., Ltd., diameter2 , ultrasonic frequency is 40kHz) for 1 hour to form a composite suspension of the two, take 6 μL and apply it on a glassy carbon substrate electrode of 0.07 square centimeters, and dry it at room temperature for 30 minutes to obtain a polyaniline / carbon nanotube composite electrode .

[0029]At the same time, the catalytic effect of the electrode on nicotinamide adenine dinucleotide (NADH, reduced form) was tested, wherein the solution used in the test was a phosphate buffer solution pH=7, and the results are shown in figure 2 . figure 2 The middle dotted line and the solid line are the cyclic voltammetry curves of the polyaniline / carbon nanotube composite electrode without and with 4mM nicotinamide adenine dinucleotide (NADH, reduced form), an...

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Abstract

The invention discloses a dehydrogenase electrode, and a preparation method and application thereof. The dehydrogenase electrode comprises a substrate electrode, a catalyst layer coated on the substrate electrode, and a dehydrogenase layer coated on the catalyst layer, wherein the catalyst layer is the compound of polyaniline and carbon nano material; and the dehydrogenase layer comprises (a) dehydrogenase or (b) dehydrogenase cross-linked by cross-linking agent. The dehydrogenase electrode can be used as an electrode of a biological fuel cell, and has high electrochemical reversibility in neutral solution; and meanwhile, in buffer solution (pH value is 7) with NAD+ (nicotinamide adenine dinucleotide, oxidation), different dehydrogenases can be used for respectively realizing selective electrochemical sensing of glucose, ethanol, lactic acid and the like.

Description

technical field [0001] The invention relates to a dehydrogenase electrode and its preparation method and application. Background technique [0002] Biofuel cells are a hot spot in scientific research in recent years, because they can operate under normal temperature and mild medium conditions, and the efficiency and specificity of the catalytic substrate of biocatalysts are better than those of platinum catalysts in conventional fuel cells. It has potential application value in bioelectronic devices such as devices, neuromorphic circuits, and sewage treatment. [0003] However, there are many key problems in the transition of enzyme-based biofuel cells from scientific theory to practical electronic products, such as short life, many interfering substances, and low power density. Studies have shown that one of the methods to solve the low power density is to increase the working potential of the battery, that is, to reduce the overpotential of cathode oxygen reduction and an...

Claims

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Application Information

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IPC IPC(8): H01M4/86H01M4/90H01M4/88H01M8/16G01N27/327
CPCY02E60/527Y02E60/50
Inventor 毛兰群周豪杰林雨青
Owner INST OF CHEM CHINESE ACAD OF SCI
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