Co-loaded adriamycin and siRNA (small interfering ribose nucleic acid) carrier capable of removing pegylation and synthesis method thereof
A technology of PEGylation and synthesis method, which is applied in the field of co-loading doxorubicin and siRNA carrier and its synthesis, can solve the problems such as inability to exert therapeutic effect, improve drug effect, avoid organic solvent residue, and increase drug effective concentration Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] 1) Synthesis of cystaminated methoxypolyethylene glycol:
[0043] Dissolve dry methoxy-terminated polyethylene glycol (abbreviated as mPEG) with a molecular weight of 800Da, dibutyltin dilaurate and 2,2'-dithiodiethylisocyanate in anhydrous toluene, under nitrogen atmosphere React at 85°C for 48 hours, then precipitate in anhydrous n-hexane for 3 times, and dry the obtained solid in vacuum to obtain 2,2'-dithiodiethylisocyanate-modified mPEG; wherein, the added mPEG 1. The molar ratio of dibutyltin dilaurate to 2,2'-dithiodiethylisocyanate is 1:0.02:4, and 10g of mPEG is dissolved in every 100ml of anhydrous toluene;
[0044] Dissolve 2,2'-dithiodiethylisocyanate-modified mPEG in distilled water, then react at 60°C for 6 hours, and finally dialyze and freeze-dry the obtained reaction solution to obtain white powder cystamination Methoxy-terminated polyethylene glycol (abbreviated as cystaminated mPEG); wherein, 5 g of 2,2'-dithiodiethylisocyanate-modified mPEG was diss...
Embodiment 2
[0060] 1) Synthesis of cystaminated methoxypolyethylene glycol:
[0061] Dissolve dry methoxy-terminated polyethylene glycol (abbreviated as mPEG) with a molecular weight of 2000Da, dibutyltin dilaurate and 2,2'-dithiodiethylisocyanate in anhydrous toluene, under nitrogen atmosphere React at 85°C under a nitrogen atmosphere for 48 hours, then precipitate in anhydrous n-hexane for 3 times, and dry the obtained solid in vacuum to obtain 2,2'-dithiodiethylisocyanate-modified mPEG; wherein, the added The molar ratio of mPEG, dibutyltin dilaurate and 2,2'-dithiodiethylisocyanate is 1:0.04:4; 20g of mPEG is dissolved in 100ml of anhydrous toluene;
[0062] Dissolve 2,2'-dithiodiethylisocyanate-modified mPEG in distilled water, then react at 60°C for 6 hours, and finally dialyze and freeze-dry the obtained reaction solution to obtain white powder cystamination Methoxy-terminated polyethylene glycol (abbreviated as cystaminated mPEG); wherein, 15 g of 2,2'-dithiodiethylisocyanate-mod...
Embodiment 3
[0074] 1) Synthesis of cystaminated methoxypolyethylene glycol:
[0075] Dissolve dry methoxy-terminated polyethylene glycol (abbreviated as mPEG) with a molecular weight of 2000Da, dibutyltin dilaurate and 2,2'-dithiodiethylisocyanate in anhydrous toluene, under nitrogen atmosphere React at 85°C under a nitrogen atmosphere for 48 hours, then precipitate in anhydrous n-hexane for 3 times, and dry the obtained solid in vacuum to obtain 2,2'-dithiodiethylisocyanate-modified mPEG; wherein, the added The molar ratio of mPEG, dibutyltin dilaurate and 2,2'-dithiodiethylisocyanate is 1:0.05:5; 10g of mPEG is dissolved in 100ml of anhydrous toluene;
[0076] Dissolve 2,2'-dithiodiethylisocyanate-modified mPEG in distilled water, then react at 60°C for 6 hours, and finally dialyze and freeze-dry the obtained reaction solution to obtain white powder cystamination Methoxy-terminated polyethylene glycol (abbreviated as cystaminated mPEG); wherein, 10 g of 2,2'-dithiodiethylisocyanate-mod...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com