High-throughput multi-channel magnetic sensing identification method for low-abundance biomolecules
A technology of biomolecules and identification methods, applied in the field of biomolecular identification, can solve the problems of high price, cross-activity interference, unable to meet the rapid early diagnosis of major diseases, large-scale screening of food safety, etc.
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Embodiment 1
[0049] Example 1: Medical application: detection of breast cancer marker molecule CEA
[0050] The schematic diagram of the whole identification method can be as follows figure 1 , which shows the connection modification methods before each device, antibody, and antigen; the whole step process, such as figure 2 shown;
[0051] Step 1 Synthesis and surface modification of magnetic nanoparticles:
[0052] In order to improve the chemical stability and biocompatibility of magnetic particles, considering the three-dimensional space structure, hydrogen bond, electrostatic force and hydrophobic effect, the present invention adopts wrapping inert metal, high molecular polymer and silica shell on the surface of magnetic nanoparticles Direct surface modification technology enables the surface of magnetic nanoparticles to have chemical stability and excellent biocompatibility. In this example, Fe 3 o 4 Take magnetic particles as an example, the specific method is to weigh 1.2gFeC...
Embodiment 2
[0073] Example 2: Food safety application: detection of aflatoxin (AFB1) content in rice
[0074] Synthesis and surface modification of the first step of magnetic nanoparticles: the same as the first step of Example 1.
[0075] Step 2, surface biofunctionalization of magnetic particles: same as Step 2 of Example 1.
[0076] Step 3, fabrication of the magnetic quantum sensor array: the same as the third step of Embodiment 1, but the size of the sensor array is 50x50 micrometers (μm).
[0077] Step 4, surface modification of the magnetic quantum sensor: same as Step 4 of Example 1.
[0078] Step 5, the chemical method of binding the capture antibody to the sensor surface: use a pipette to drip 50nL of AFB1 antigen antibody on the sensor surface (purchased in the United States, Sigma–Aldrich, St.Louis, MO), in the control sensor 50mL of bovine serum albumin (bovineserumalbumin, BSA) was dripped into part with a pipette. The sensor chip was then left at 4°C for 12 hours and fin...
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