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Method for preparing Inonotus obliquus crude polysaccharide by using d101 resin

A kind of technology of Inonotus obliquus and crude polysaccharide, which is applied in the field of preparing Inonotus obliquus crude polysaccharide

Inactive Publication Date: 2016-05-25
横琴智库转化医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Macroporous resin used to remove crude polysaccharides from Inonotus obliquus has not been reported in the literature

Method used

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  • Method for preparing Inonotus obliquus crude polysaccharide by using d101 resin
  • Method for preparing Inonotus obliquus crude polysaccharide by using d101 resin
  • Method for preparing Inonotus obliquus crude polysaccharide by using d101 resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Utilize D101 resin to prepare the method for crude polysaccharide of Inonotus obliquus, comprises the steps:

[0019] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak in 4 times the volume of 60% ethanol solution for 16 hours, to remove fat, some mono (oligo) sugars and fat-soluble pigments, and place the residue in a cool place Air dry.

[0020] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 10 times distilled water (v / w), extract in boiling water for 1 hour, filter with 80-mesh gauze, concentrate the filtrate, centrifuge, and spray-dry to obtain crude polysaccharide.

[0021] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water, prepare a 0.5% (w / w) aqueous solution, add papain, the amount of papain added is 3% (w / w) of the crude polysaccharide aqueous solution, and react at 35°C for 20 minutes to o...

Embodiment 2

[0025] Example 2 Utilize D101 resin to prepare the method for crude polysaccharide of Inonotus obliquus, comprises the steps:

[0026] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak in 8 times the volume of 95% ethanol solution for 24 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and place the residue in a cool place Air dry.

[0027] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 20 times distilled water (v / w), extract with boiling water for 3 hours, filter with 120-mesh gauze, repeat the extraction of the filter residue twice, combine the filtrate, concentrate, centrifuge, and spray dry get crude polysaccharides.

[0028] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 2.0% (w / w) aqueous solution, add papain, the amount of papain added is 5% (w / w) of the crude polysacch...

Embodiment 3

[0033] Example 3 Utilize D101 resin to prepare the method for crude polysaccharide of Inonotus obliquus, it comprises the steps:

[0034] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak in 7 times the volume of 90% ethanol solution for 20 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and place the residue in a cool place Air dry.

[0035] B. Preparation of crude polysaccharide by hot water leaching: After the pretreated residue is air-dried, add 18 times distilled water (v / w), extract with boiling water for 2.5 hours, filter with 100-mesh gauze, repeat the extraction of the filter residue 3 times, combine the filtrate, concentrate, centrifuge, and spray Dried crude polysaccharides.

[0036] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 1.8% (w / w) aqueous solution, add papain, the amount of papain added is 4.5% (w / w) of the crude polysa...

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Abstract

The invention discloses a method for preparing crude polysaccharides from Inonotus obliquus by using D101 resin, which comprises the following steps: A. Degreasing pretreatment of raw materials; B. Preparation of crude polysaccharide by hot water extraction; C. Crude polysaccharide enzymatic hydrolysis; D. Macroporous adsorption resin D101 treatment; E. Ultrafiltration to remove small molecules; F. Vacuum freeze-drying; preparation of Inonotus obliquus polysaccharides. Using this process, the refined polysaccharides of Inonotus obliquus that are decolorized and remove most of the protein and small molecular impurities are finally obtained, which have high polysaccharide retention rate, high decolorization rate and high protein removal rate, and are suitable for industrial scale production of chaga Poria refined polysaccharides.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing crude polysaccharides from Inonotus obliquus by using D101 resin. Background technique [0002] Inonotus obliquus[ Inonotus obliquus (Fr.) Pilat] is a famous folk wild medicinal fungus. Studies have shown that Inonotus obliquus polysaccharide is one of the important active ingredients, which has various pharmacological activities such as enhancing immune regulation, anti-tumor, anti-oxidation, lowering blood sugar and anti-virus, and has become one of the hot research fields at home and abroad. However, there are relatively few reports on the isolation and purification of polysaccharides from Inonotus obliquus. [0003] Due to the complexity of the composition of polysaccharides, the molecular weight distribution of the crude polysaccharide extract is wide, and there are many types of polysaccharides, and previous experiments have shown that the e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 施璐
Owner 横琴智库转化医学有限公司