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Method for efficiently and rapidly removing protein and pigment from the polysaccharide extract of Acanthopanax polysaccharides

A technology of Acanthopanax polysaccharide and extract, applied in the field of extraction and purification of natural products

Active Publication Date: 2021-08-20
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, in the current existing technology, after adding protease to the extract to degrade the protein molecules, the protein molecules and pigment molecules in it are removed together with the reverse micellar solution technology, so as to realize the high-efficiency removal of protein and pigment impurities. Routing

Method used

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  • Method for efficiently and rapidly removing protein and pigment from the polysaccharide extract of Acanthopanax polysaccharides
  • Method for efficiently and rapidly removing protein and pigment from the polysaccharide extract of Acanthopanax polysaccharides
  • Method for efficiently and rapidly removing protein and pigment from the polysaccharide extract of Acanthopanax polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1) Grind the dried orangiosus bark, pass through a 60-mesh sieve, add 95% volume fraction of ethanol solution to the sieved coarse powder according to the ratio of 1g:10ml to liquid, reflux at 40°C for 1 hour, and remove the fat and other components, filtered under reduced pressure, the ethanol solvent in the filter cake was evaporated to dryness, and the powder was recovered for later use.

[0067] 2) Mix the degreased dry Antsotosa powder with deionized water at a ratio of 1g:12ml, soak at 60°C for 4h, and then perform reflux extraction twice at 95°C for 15min. Combine and collect the extracts for 60 minutes each time; centrifuge at 8000 rpm for 20 minutes, collect the supernatant and concentrate in vacuum to the same weight as the original medicinal material, add 4.5 times the volume of 95% ethanol to the concentrated filtrate, carry out alcohol precipitation at room temperature for 24 hours, and filter with suction , the supernatant after alcohol precipitation is co...

Embodiment 2

[0075] Comparison of Protein Removal Efficiency of Different Proteases

[0076] Add papain, neutral protease and alkaline protease of the same activity unit respectively, carry out under enzymatic reaction conditions, other conditions are all carried out according to embodiment 1 step, calculate the egg removal rate under each ratio and the retention rate of polysaccharide, the result See Table 1.

[0077] The comparison of different proteases in table 1 to protein removal rate

[0078]

[0079] As can be seen from Table 1, under the same amount of enzymes, papain has the best deproteinization effect, with a protein removal rate of 88.29%; followed by alkaline protease, with a protein removal rate of 71.64%; neutral protease deproteinization The effect is not ideal. Considering the ratio of protein removal rate to polysaccharide loss rate among the three, papain was the best.

Embodiment 3

[0081] Effect of enzymatic hydrolysis time on protein removal rate

[0082] According to the method of Example 1, the enzymolysis time was adjusted to 0.5-3.5h, and other conditions were kept constant, and the protein and pigment removal rate and polysaccharide retention rate were calculated at each enzymolysis time. The results showed that the protein removal rate increased rapidly within 2 hours with the prolongation of enzymatic hydrolysis time, reached a regional peak at 1.5-2 hours, and showed a downward trend after 2 hours.

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Abstract

The invention provides a method for deproteinizing and depigmenting the polysaccharide extract of Acanthopanax polysaccharides. After adding protease to the extract to degrade the protein molecules, the protein molecules and pigment molecules are removed together by means of reverse micellar solution treatment technology. Synchronous and high-efficiency removal of protein and pigment impurities is achieved through the joint treatment of protease-CTAB. Wherein, the reverse micelles treatment solution includes cetyltrimethylammonium bromide (CTAB) and a composite solvent; the composite solvent is composed of an organic solvent and water.

Description

technical field [0001] The invention relates to the field of extraction and purification of natural products, in particular to a method for deproteinizing and depigmenting the polysaccharide extract of Acanthopanax polysaccharides. Background technique [0002] Polysaccharide, also known as polysaccharide, is a natural polymer compound composed of more than 20 to tens of thousands of monosaccharides, which widely exists in animals, plants, fungi and microorganisms. As a natural active ingredient, polysaccharide is favored by researchers at home and abroad because of its various biological activities, non-toxic, harmless, no residue, and no drug resistance. It is a hot spot in the fields of medicine, agriculture, and food research. The biological activity of polysaccharides has an important relationship with its purity and structure. However, like the extraction of other natural active substances, it faces problems such as complex raw material components and low content of ta...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陈永吴江梁淑娟
Owner WEIFANG MEDICAL UNIV
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