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Preparation process of β-glucan

A preparation process and glucan technology, applied in the field of preparation technology of beta-glucan, can solve the problems of low purity of finished products, decolorization, large loss of deproteinization steps, poor use effect, etc.

Active Publication Date: 2021-06-04
SHANGHAI JAKA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nowadays, the production of β-glucan has entered a large scale, but the purity of the finished product prepared by the existing production process is low, and the decolorization and deproteinization steps in the production process are lossy, and the use effect is poor

Method used

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preparation example Construction

[0045] A preparation process of β-glucan, comprising the following steps:

[0046] S1: strain screening:

[0047] Prepare the strains, carry out the Congo red agar staining method qualitative test respectively, detect the β-1,3-glucanase activity of the strains, carry out screening, and obtain the strains to be cultured, and set aside;

[0048] S2: strain culture;

[0049] Take the strain to be cultured, culture the slant seeds for 7-8 days at a culture temperature of 26-28°C, inoculate them on a plate medium, and cultivate them for 7-8 days under the conditions of 25-30°C and 170-180r / min to obtain a seed liquid;

[0050] S3: Fermentation:

[0051] Take the seed liquid, put it in a fermenter for 7-8 days, and stir at a speed of 100-300r / min to obtain a fermented liquid; add starch debranching enzyme and glucoamylase to the fermenter in sequence after 3-4 days of fermentation in the process, and the temperature is 28-300r / min. Carry out enzymatic hydrolysis at 32°C;

[005...

Embodiment 1

[0060] S1: strain screening:

[0061] Prepare the strains, carry out the Congo red agar staining method qualitative test respectively, detect the β-1,3-glucanase activity of the strains, carry out screening, and obtain the strains to be cultured, and set aside;

[0062] S2: strain culture;

[0063] Take the strain to be cultured, culture the slant seeds for 7 days at a culture temperature of 26°C, inoculate them on a plate medium, and cultivate them for 7 days at 25°C and 170r / min to obtain a seed solution;

[0064] S3: Fermentation:

[0065] Take the seed liquid, put it in a fermenter for 7 days, and stir at a speed of 100r / min to obtain a fermented liquid; add starch debranching enzyme and glucoamylase to the fermenter in sequence after 3 days of fermentation during the process, and carry out enzymatic hydrolysis at a temperature of 28°C ;

[0066] S4: Rough extraction and decolorization:

[0067] The fermentation broth was irradiated with γ-rays, and the irradiation sou...

Embodiment 2

[0074] S1: strain screening:

[0075] Prepare the strains, carry out the Congo red agar staining method qualitative test respectively, detect the β-1,3-glucanase activity of the strains, carry out screening, and obtain the strains to be cultured, and set aside;

[0076] S2: strain culture;

[0077] Take the strain to be cultured, culture the slant seeds for 7 days at a culture temperature of 27°C, inoculate them on a plate medium, and cultivate them for 7 days at 28°C and 175 r / min to obtain a seed liquid;

[0078] S3: Fermentation:

[0079] Take the seed liquid, put it in a fermenter for 7 days, and stir at a speed of 200r / min to obtain a fermented liquid; add starch debranching enzyme and glucoamylase to the fermenter in sequence after 4 days of fermentation during the process, and carry out enzymatic hydrolysis at a temperature of 30°C ;

[0080] S4: Rough extraction and decolorization:

[0081] The fermentation broth was irradiated with γ-rays, and the irradiation sour...

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Abstract

The invention discloses a preparation process of β-glucan. Firstly, strains are screened, several strains of strains are prepared, and qualitatively detected by Congo red agar staining method to detect the β-1,3-glucan of each strain. Dextranase activity, screening strains with higher enzyme activity as strains to be cultivated. Then the strains are cultivated, first cultivated by slant seeds for 7-8d, and then inoculated to the plate medium for cultivation, wherein the components of the plate medium include glucose 28-30g / L, potassium dihydrogen phosphate 1-2g / L, Yeast extract 6‑10g / L, agar 18‑20g / L, magnesium sulfate 1‑2g / L, ammonium chloride 2‑3g / L. The application discloses a preparation process of β-glucan, the process design is reasonable, and the operation is simple. The application prepares β-glucan by fermenting, separating and purifying Schizophyllum fungus, and optimizing and improving various parameter steps. The product has good water solubility, high biological activity, and high yield, retention rate and purity of crude sugar, can be applied to large-scale production, has low cost, and has high practicability.

Description

technical field [0001] The invention relates to the technical field of beta-glucan preparation, in particular to a preparation process of beta-glucan. Background technique [0002] Glucan refers to a homopolysaccharide composed of glucose as a monosaccharide, and the glucose units are connected by glycosidic bonds. According to the type of glycosidic bonds, it can be divided into alpha-glucan and beta-glucan. [0003] The beta-glucan (beta-glucan) active structure is a polysaccharide composed of glucose units, most of which are bound by beta-1,3, which is how the glucose chains are linked. It can activate macrophages, neutrophils, etc., so it can increase the content of leukin, cytokinin and special antibodies, and fully stimulate the body's immune system; at the same time, β-glucan also has the functions of removing free radicals, anti-radiation, Dissolving cholesterol, preventing hyperlipidemia, etc., can be widely used in medicine, food, cosmetics and other fields. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/08C12N1/14C08B37/02C12R1/645
CPCC08B37/0003C08B37/0024C12N1/14C12P19/08
Inventor 田军刘青卢伊娜
Owner SHANGHAI JAKA BIOTECH CO LTD