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Method for refining jelly jelly polysaccharide by adopting ammonium sulfate and ctab precipitation combined with macroporous resin

A technology of macroporous resin and jelly jelly, which is applied in the field of refining and processing plant active ingredients, can solve the problems of cumbersome operation, affecting the biological activity of polysaccharides, and difficult removal of activated carbon powder, and achieve the effect of high polysaccharide retention rate

Active Publication Date: 2020-07-14
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the jelly jelly polysaccharide extracted from jelly jelly has a high protein content and is dark brown, which not only affects the appearance of the polysaccharide as a food additive or functional food base, but also has a great impact on the subsequent purification of the polysaccharide , so it is very necessary for the refining of jelly polysaccharide
The traditional polysaccharide deproteinization method is based on the Sevage method, which is cumbersome to operate and low in efficiency. The organic solvent used is toxic and affects the biological activity of polysaccharides. It is necessary to continue to explore a new polysaccharide refining method to maximize its utilization value

Method used

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  • Method for refining jelly jelly polysaccharide by adopting ammonium sulfate and ctab precipitation combined with macroporous resin
  • Method for refining jelly jelly polysaccharide by adopting ammonium sulfate and ctab precipitation combined with macroporous resin
  • Method for refining jelly jelly polysaccharide by adopting ammonium sulfate and ctab precipitation combined with macroporous resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Utilize ammonium sulfate and CTAB precipitation to combine the method for D301 macroporous resin to refine jelly jelly polysaccharide, comprising steps as follows:

[0023] A. Pretreatment of raw materials: after pulverizing the jelly grass, soak it with 8 times the volume of 60% ethanol for 16 hours, and air-dry the residue in a cool place.

[0024] B. Preparation of crude polysaccharide by hot water leaching: add 10 times the volume of aqueous solution to the pretreated jelly grass, extract with boiling water for 1 hour, filter the supernatant with 120-mesh gauze, repeat the extraction of the filter residue once, combine the filtrate, concentrate, and centrifuge to obtain Crude polysaccharide solution of jelly grass.

[0025] C. Precipitation with ammonium sulfate: Precipitate the crude polysaccharide solution of G. jellia with saturated ammonium sulfate solution at 4° C. for 8 hours, and the volume ratio of polysaccharide and ammonium sulfate solution is 1:1. The pr...

Embodiment 2

[0031] Utilize ammonium sulfate and CTAB precipitation to combine the method for D301 macroporous resin to refine jelly jelly polysaccharide, comprising steps as follows:

[0032] A. Pretreatment of raw materials: After crushing the jelly grass, soak it with 9 times the volume of 80% ethanol for 24 hours, and air-dry the residue in a cool place.

[0033] B, hot water leaching to prepare crude polysaccharides: add 15 times the volume of aqueous solution to the pretreated jelly grass, extract with boiling water for 2 hours, filter the supernatant with 120 mesh gauze, repeat the extraction of the filter residue twice, combine the filtrates, concentrate, and centrifuge to obtain Crude polysaccharide solution of jelly grass.

[0034] C. Precipitation with ammonium sulfate: the crude polysaccharide solution of A. japonicus was precipitated with saturated ammonium sulfate solution at 4° C. for 10 h, and the volume ratio of polysaccharide and ammonium sulfate solution was 1:1. The pr...

Embodiment 3

[0040] Utilize ammonium sulfate and CTAB precipitation to combine the method for D301 macroporous resin to refine jelly jelly polysaccharide, comprising steps as follows:

[0041] A. Pretreatment of raw materials: After the jelly grass is crushed, it is soaked with 10 times the volume of 95% ethanol for 24 hours, and the residue is air-dried in a cool place.

[0042] B, hot water leaching to prepare crude polysaccharides: add 20 times the volume of aqueous solution to the pretreated jelly grass, extract with boiling water for 3 hours, filter the supernatant with 120 mesh gauze, repeat the extraction of the filter residue 3 times, combine the filtrates, concentrate, and centrifuge to obtain Crude polysaccharide solution of jelly grass.

[0043] C. Precipitation with ammonium sulfate: Precipitate the crude polysaccharide solution of G. jellia with saturated ammonium sulfate solution at 4° C. for 12 hours, and the volume ratio of polysaccharide and ammonium sulfate solution is 1:...

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Abstract

The invention relates to the field of plant active component refining treatment methods, in particular to a method for refining mesona chinensis benth polysaccharide by combination of ammonium sulfateand CTAB (cetyltrimethyl ammonium bromide) precipitates and macroporous resin. According to the method, mesona chinensis benth is pretreated and extracted to obtain crude polysaccharide, and polysaccharide is refined by a method of combining the ammonium sulfate and CTAB precipitates and the D301 macroporous resin. After the mesona chinensis benth polysaccharide is treated by the method, residuesof protein and small-molecular impurities can be greatly reduced, product purity is improved, higher polysaccharide retention rate, color removal rate, protein removal rate and yield is acquired, andbiological activity of the polysaccharide can be effectively retained. Provided optimization conditions are low in cost, simple to operate, good in effect and applicable to industrial production, anddamage to the mesona chinensis benth polysaccharide is less.

Description

technical field [0001] The invention belongs to the field of refining and processing methods of plant active ingredients, and in particular relates to a method for refining jelly jelly polysaccharides by using ammonium sulfate and CTAB precipitation combined with D301 macroporous resin. Background technique [0002] Jelly grass, also known as jelly grass, is an edible and medicinal annual herb. In China, it has been used as an important medicinal and edible plant resource, often as a food ingredient in the production of herbal teas and jam-type desserts and edible gels. It has many biological activities including anti-oxidation, heat stroke protection, liver protection, anti-hypertension and hypoglycemic effects. Polysaccharides are one of the main components of jelly grass. Studies have shown that jelly jelly polysaccharides are composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, and galacturonic acid. Jelly jelly polysaccharide can be compounded with ot...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 谢建华黄莉鑫申明月黄志兵王文洁
Owner NANCHANG UNIV