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Preparation process of beta-glucan

A preparation process and glucan technology, applied in the field of preparation technology of beta-glucan, can solve the problems of low purity of finished products, poor use effect, decolorization, large loss of deproteinization steps, etc., and achieve high biological activity and cost. Low, high retention and purity effects

Active Publication Date: 2020-10-16
SHANGHAI JAKA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nowadays, the production of β-glucan has entered a large scale, but the purity of the finished product prepared by the existing production process is low, and the decolorization and deproteinization steps in the production process are lossy, and the use effect is poor

Method used

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preparation example Construction

[0045] A preparation process of β-glucan, comprising the following steps:

[0046] S1: strain screening:

[0047] Prepare the strains, carry out the Congo red agar staining method qualitative test respectively, detect the β-1,3-glucanase activity of the strains, carry out screening, and obtain the strains to be cultured, and set aside;

[0048] S2: strain culture;

[0049] Take the strain to be cultured, culture the slant seeds for 7-8 days at a culture temperature of 26-28°C, inoculate them on a plate medium, and cultivate them for 7-8 days under the conditions of 25-30°C and 170-180r / min to obtain a seed liquid;

[0050] S3: Fermentation:

[0051] Take the seed liquid, put it in a fermenter for 7-8 days, and stir at a speed of 100-300r / min to obtain a fermented liquid; add starch debranching enzyme and glucoamylase to the fermenter in sequence after 3-4 days of fermentation in the process, and the temperature is 28-300r / min. Carry out enzymatic hydrolysis at 32°C;

[005...

Embodiment 1

[0060] S1: strain screening:

[0061] Prepare the strains, carry out the Congo red agar staining method qualitative test respectively, detect the β-1,3-glucanase activity of the strains, carry out screening, and obtain the strains to be cultured, and set aside;

[0062] S2: strain culture;

[0063] Take the strain to be cultured, culture the slant seeds for 7 days at a culture temperature of 26°C, inoculate them on a plate medium, and cultivate them for 7 days at 25°C and 170r / min to obtain a seed solution;

[0064] S3: Fermentation:

[0065] Take the seed liquid, put it in a fermenter for 7 days, and stir at a speed of 100r / min to obtain a fermented liquid; add starch debranching enzyme and glucoamylase to the fermenter in sequence after 3 days of fermentation during the process, and carry out enzymatic hydrolysis at a temperature of 28°C ;

[0066] S4: Rough extraction and decolorization:

[0067] The fermentation broth was irradiated with γ-rays, and the irradiation sou...

Embodiment 2

[0074] S1: strain screening:

[0075] Prepare the strains, carry out the Congo red agar staining method qualitative test respectively, detect the β-1,3-glucanase activity of the strains, carry out screening, and obtain the strains to be cultured, and set aside;

[0076] S2: strain culture;

[0077] Take the strain to be cultured, culture the slant seeds for 7 days at a culture temperature of 27°C, inoculate them on a plate medium, and cultivate them for 7 days at 28°C and 175 r / min to obtain a seed liquid;

[0078] S3: Fermentation:

[0079] Take the seed liquid, put it in a fermenter for 7 days, and stir at a speed of 200r / min to obtain a fermented liquid; add starch debranching enzyme and glucoamylase to the fermenter in sequence after 4 days of fermentation during the process, and carry out enzymatic hydrolysis at a temperature of 30°C ;

[0080] S4: Rough extraction and decolorization:

[0081] The fermentation broth was irradiated with γ-rays, and the irradiation sour...

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Abstract

The invention discloses a preparation process of beta-glucan. The process comprises the following steps of firstly screening strains: preparing a plurality of strains, qualitatively detecting the strains by using a Congo red agar staining method, detecting the beta-1, 3-glucanase activity of each strain, and screening out the strain with the relatively high enzyme activity as a strain to be cultured; and secondly culturing the strain: performing slant seed culture for 7-8 days first, and then inoculating the strain to a plate culture medium for culture, wherein the plate culture medium comprises the following components: 28-30 g / L of glucose, 1-2 g / L of potassium dihydrogen phosphate, 6-10 g / L of yeast extract, 18-20 g / L of agar, 1-2 g / L of magnesium sulfate and 2-3 g / L of ammonium chloride. The preparation process of the beta-glucan is reasonable in design and easy to operate; the beta-glucan is prepared through fermentation, separation and purification of schizophyllum commune and optimization and improvement of various parameters and steps; the beta-glucan is good in water solubility, high in biological activity and relatively high in crude sugar yield, retention rate and purity; and the preparation process can be applied to large-scale production, is low in cost and has relatively high practicability.

Description

technical field [0001] The invention relates to the technical field of beta-glucan preparation, in particular to a preparation process of beta-glucan. Background technique [0002] Glucan refers to a homopolysaccharide composed of glucose as a monosaccharide, and the glucose units are connected by glycosidic bonds. According to the type of glycosidic bonds, it can be divided into alpha-glucan and beta-glucan. [0003] The beta-glucan (beta-glucan) active structure is a polysaccharide composed of glucose units, most of which are bound by beta-1,3, which is how the glucose chains are linked. It can activate macrophages, neutrophils, etc., so it can increase the content of leukin, cytokinin and special antibodies, and fully stimulate the body's immune system; at the same time, β-glucan also has the functions of removing free radicals, anti-radiation, Dissolving cholesterol, preventing hyperlipidemia, etc., can be widely used in medicine, food, cosmetics and other fields. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08C12N1/14C08B37/02C12R1/645
CPCC08B37/0003C08B37/0024C12N1/14C12P19/08
Inventor 田军刘青卢伊娜
Owner SHANGHAI JAKA BIOTECH CO LTD
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