Method for germinating peony seeds and promoting growth of peony seedlings by utilizing bacteria that promote peony growth and antagonize pathogenic bacteria
A technology of peony seeds and pathogenic bacteria is applied in the field of using bacteria that promote the growth of peony and antagonize pathogenic bacteria to germinate peony seeds and promote the growth of peony seedlings. And other issues
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0055] Example 1. Isolation and identification of Paenibacillus polymyxa CSM1101
[0056] 1. Isolation of Paenibacillus polymyxa CSM1101
[0057] Soil samples were collected from the rhizosphere of peony (Paeonia ostii, Fengdan) (6-year-old) plants in Jinshan Village, Jinshan Village, Shun'an Town, Tongling City, Anhui Province, and the soil samples were placed in sterile water and sterilized In a small Erlenmeyer flask with glass beads, shake at 200rpm / min for 20min; take the shaken suspension for serial gradient dilution, 10 3 、10 4 Pipette 200μl of each dilution suspension and spread evenly Nitrogen-free medium, cultured at 30°C for 4 days, picked a single colony, and placed in Nitrogen-free medium solid medium plates were purified by streaking. One of the isolated and purified strains was named CSM1101.
[0058] 2. Identification of nitrogen-fixing bacteria CSM1101 in the rhizosphere of tree peony
[0059] 1. Observation of morphological characteristics and determi...
Embodiment 2
[0086] Example 2. Biological activity of Paenibacillus polymyxa CSM1101
[0087] 1. Determination of Nitrogen Fixation Activity of Paenibacillus polymyxa CSM1101 Nitrogen fixation activity was determined by acetylene reduction method. Add 2.5mL of semi-solid to a 15mm×15mm screw-top test tube Nitrogen-free medium was inoculated with Paenibacillus polymyxa (Paenibacillus polymyxa) CSM1101CGMCC No.8527, plugged with a cotton plug, and incubated at 30°C for 24 hours; then replaced with a sterile rubber stopper, pumped out 10% of the gas, and injected the same volume of acetylene, Incubate at 30°C for 24h. The gas samples were taken to measure ARA (acetylene reduction activity, acetylene reduction activity) on a Varian VISTA6000 gas chromatograph. The experiment was repeated three times, and the mean value was taken and the variance was not greater than 5%.
[0088] The assay results showed that CSM1101 was in Nitrogen-fixing activity was higher in nitrogen-free medium. Its...
Embodiment 3
[0099] Example 3, using corn stalk enzymatic hydrolysis solution to produce bacterial agent A that promotes the growth of peony and antagonizes pathogenic bacteria
[0100] 1. Preparation of fermentation medium
[0101] Take 8000g of corn stalks (calculated by dry weight), cut them into small pieces, put them into a hydrothermal reactor, carry out hydrothermal treatment at 180°C and 1.0Mpa for 20 minutes, and then dry them naturally to obtain pretreated corn stalks; After the pretreated corn stalks were crushed to 100 mesh, cellulase was added at a ratio of 15 FPU per gram of dry straw, and distilled water was added at a ratio of 1L of distilled water per 200 grams of dry straw, at 55°C. 100rpm (radius of rotation 20mm), hydrolyzed at pH=4.8 for 48 hours, then cooled and centrifuged, and the supernatant and precipitate were collected respectively. The supernatant was the enzymatic hydrolysis solution, and the precipitate was the enzymatic hydrolysis residue. The glucose conte...
PUM
| Property | Measurement | Unit |
|---|---|---|
| length | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More