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[ru(tatp)3]2+ modified electrode and method for detecting glucose, uric acid and ascorbic acid in blood

A technology for modifying electrodes and ascorbic acid, which is applied in the field of electrochemical detection, can solve the problems of low sensitivity, not easy to carry, and poor reproducibility of electrochemical biosensors, achieve good redox activity, avoid possible inactivation, and have few side reactions Effect

Inactive Publication Date: 2016-08-31
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As one of the two main methods that can jointly detect glucose, uric acid and ascorbic acid, high performance liquid chromatography has a wide range of applications, but requires expensive and large instruments and is not easy to carry; the other method is electrochemical biosensor detection method, Electrochemical biosensors are divided into active enzyme and non-enzyme sensors. Active enzyme electrochemical biosensors have problems such as low sensitivity, poor reproducibility, high cost and difficulty in mass production.

Method used

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  • [ru(tatp)3]2+ modified electrode and method for detecting glucose, uric acid and ascorbic acid in blood
  • [ru(tatp)3]2+ modified electrode and method for detecting glucose, uric acid and ascorbic acid in blood
  • [ru(tatp)3]2+ modified electrode and method for detecting glucose, uric acid and ascorbic acid in blood

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Embodiment 1

[0036] A kind of [Ru(tatp) of the present embodiment 3 ] 2+ The modified electrode is prepared by the following preparation method:

[0037] Dissolve [Ru(tatp) in DMSO 3 ]Cl 2 Prepare 0.5mmol / L of [Ru(tatp) 3 ] 2+solution, and then 20μL of the above [Ru(tatp) 3 ] 2+ Add the solution dropwise onto the graphite electrode, put the graphite electrode into an oven and dry it at a constant temperature at 40-80°C to obtain [Ru(tatp) 3 ] 2+ Modified electrodes.

[0038] [Ru(tatp) of the present embodiment 3 ]Cl 2 Through literature (South China Normal University 2012 Ji Shibo master's degree thesis "DNA and BSA promote ruthenium (II) complexes in ITO and nano-TiO 2 The preparation method described in "Assembly on Particles and Photoelectric Performance Research" p30) is prepared, and the preparation process is as follows: 0.065g (0.25mmol) RuCl 3 ·3H 2 O, 0.116g (0.5mmol) tatp (1,4,8,9-tetraazol terphenyl), 1.5mL 37% concentrated hydrochloric acid and 1.5mL pure water wer...

Embodiment 2

[0051] [Ru(tatp) of embodiment 1 3 ] 2+ The modified electrode is used to detect the method for glucose, uric acid and ascorbic acid in human blood samples, and the specific steps are as follows:

[0052] (1) [Ru(tatp) of embodiment 1 3 ] 2+ The modified electrode, the counter electrode and the reference electrode are respectively connected to the corresponding electrodes of the electrochemical workstation to construct an electrolytic cell with a three-electrode system, and then add 10 mmol / L tris buffer solution in the tank of the electrolytic cell, Use cyclic voltammetry to perform 15 cycles of cyclic scanning activation in the potential range of 0-1.2V;

[0053] (2) Use the potential step method to keep the potential at 0.3V. When the current tends to be stable, add 10 μL of the blood sample to be tested dropwise into the electrolytic cell to obtain the response current of glucose in the blood, and compare the response current with the glucose concentration-response curr...

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Abstract

The invention relates to the technical field of electrochemical detection, and discloses a [Ru(tatp)3]<2+> modified electrode and a method for detecting glucose, uric acid and ascorbic acid in blood by adopting electrode. The [Ru(tatp)3]<2+> modified electrode is obtained by dropping a [Ru(tatp)3]<2+> solution on a work electrode and then performing constant-temperature drying at a temperature of 40-80 DEG C, wherein the solution is prepared by dissolving dimethyl sulfoxide. The method for detecting the glucose, the uric acid and the ascorbic acid in the blood by adopting the [Ru(tatp)3]<2+> modified electrode comprises the steps: connecting the [Ru(tatp)3]<2+> modified electrode into an electrochemical work station, respectively enabling potentials to be 0.25-0.35 V, 0.65-0.75 V and 0.95-1.05 V by using a potential step jump method, then dropping a blood sample, measuring response currents corresponding to different potentials, and respectively comparing with respective concentration-current standard curves, and calculating actual concentrations of the glucose, the uric acid and the ascorbic acid in the blood.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection, in particular to a [Ru(tatp) 3 ] 2+ Modified electrodes and methods for their use in the detection of glucose, uric acid and ascorbic acid in blood. Background technique [0002] Glucose, uric acid and ascorbic acid usually co-exist in human blood and play a very important role in the physiological metabolism of the human body. Among these three components, the abnormal concentration of any one will cause serious harm to human health. [0003] First, excessive glucose concentration in the blood leads to diabetes, which is a metabolic disease caused by defective insulin secretion or impaired biological action. It will cause different degrees of functional damage or obstacles to various tissues in the human body. Therefore, effective in vitro blood glucose measurement and early screening for diabetic patients are of great significance in the active prevention and treatment of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 卢烁建纪式波李红
Owner SOUTH CHINA NORMAL UNIVERSITY
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